Chronic exposure to arsenic, LINE-1 hypomethylation, and blood pressure: a cross-sectional study in Bangladesh.
Chronic exposure to arsenic, LINE-1 hypomethylation, and blood pressure: a cross-sectional study in Bangladesh.
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DOI：
10.1186/s12940-017-0231-7
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发表时间：
2017-03-07
期刊：
影响因子：
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通讯作者：
Nohara K
中科院分区：
文献类型：
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作者：
Hossain K;Suzuki T;Hasibuzzaman MM;Islam MS;Rahman A;Paul SK;Tanu T;Hossain S;Saud ZA;Rahman M;Nikkon F;Miyataka H;Himeno S;Nohara K
长期接触砷与癌症和高血压有关。越来越多的证据表明，改变甲基化在长散布核元素-1（LINE-1）参与了许多类型的疾病，包括心血管疾病。在这里，我们评估了砷暴露和LINE-1甲基化水平之间的关系，特别是与血压（BP）的关系。在孟加拉国农村地区共招募了236名受试者（175名来自砷流行地区，61名来自非流行地区）。受试者的砷暴露水平（即，饮用水、头发和指甲砷浓度）通过电感耦合等离子体质谱法测量。受试者的LINE-1甲基化水平通过焦磷酸测序确定。病区人群LINE-1甲基化水平显著低于非病区人群（p < 0.01）。在性别分层分析中，女性而非男性受试者的砷暴露水平与LINE-1甲基化水平呈显著负相关，（水砷：P < 0.01，发砷：P < 0.05，甲砷：P < 0.001）（水砷：p < 0.01，发砷：p < 0.05，指甲砷：p < 0.001）校正年龄、体重指数和吸烟。研究砷水平、BP和LINE-1甲基化之间相互作用的分析表明，砷相关的BP水平升高与LINE-1低甲基化相关。我们的研究结果表明，慢性砷暴露与血液白细胞DNA中LINE-1甲基化水平呈负相关，这在女性中比男性更明显;此外，LINE-1甲基化水平的降低可能与砷诱导的血压升高有关。本文的在线版本（doi：10.1186/s12940-017-0231-7）包含补充材料，可供授权用户使用。
Chronic exposure to arsenic is associated with cancer and hypertension. Growing evidence suggests that altered methylation in long interspersed nuclear element-1 (LINE-1) is involved in many types of disorders, including cardiovascular disease. Here we evaluated the association between arsenic exposure and LINE-1 methylation levels, especially in relation to blood pressure (BP). A total of 236 subjects (175 from arsenic-endemic areas and 61 from a non-endemic area) in rural Bangladesh were recruited. The subjects’ arsenic exposure levels (i.e., drinking water, hair and nail arsenic concentrations) were measured by inductively coupled plasma mass spectroscopy. The subjects’ LINE-1 methylation levels were determined by pyrosequencing. The average LINE-1 methylation levels of the subjects living in the arsenic-endemic areas were significantly (p < 0.01) lower than those of the subjects living in the non-endemic area. In a sex-stratified analysis, the arsenic exposure levels in female but not male subjects showed a significant inverse association with LINE-1 methylation levels before (water arsenic: p < 0.01, hair arsenic: p < 0.05, nail arsenic: p < 0.001) and after (water arsenic: p < 0.01, hair arsenic: p < 0.05, nail arsenic: p < 0.001) adjustment for age, body mass index and smoking. Analyses examining interactions among arsenic levels, BP and LINE-1 methylation showed that arsenic-related elevated levels of BP were associated with LINE-1 hypomethylation. Our findings demonstrated that chronic exposure to arsenic was inversely associated with LINE-1 methylation levels in blood leukocyte DNA and this was more pronounced in females than males; in addition, the decreased levels of LINE-1 methylation might be involved in the arsenic-induced elevation of BP. The online version of this article (doi:10.1186/s12940-017-0231-7) contains supplementary material, which is available to authorized users.