MOLECULAR IDENTITY, DISTRIBUTION AND HETEROGENEITY OF GLIAL FIBRILLARY ACIDIC PROTEIN - AN IMMUNOBLOTTING AND IMMUNOHISTOCHEMICAL STUDY OF SCHWANN-CELLS, SATELLITE CELLS, ENTERIC GLIA AND ASTROCYTES
MOLECULAR IDENTITY, DISTRIBUTION AND HETEROGENEITY OF GLIAL FIBRILLARY ACIDIC PROTEIN - AN IMMUNOBLOTTING AND IMMUNOHISTOCHEMICAL STUDY OF SCHWANN-CELLS, SATELLITE CELLS, ENTERIC GLIA AND ASTROCYTES
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DOI：
10.1007/bf01148114
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
MIRSKY, R
中科院分区：
文献类型：
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作者：
JESSEN, KR;THORPE, R;MIRSKY, R
胶质细胞酸性蛋白[GFAP]被确定为星形胶质细胞中间丝的主要成分。在肠神经系统的神经胶质细胞和PNS [外周神经系统]的一些许旺细胞中也检测到它。PNS中GFAP免疫反应性的分子身份迄今尚未研究。本研究比较了GFAP在成年大鼠CNS和PNS的免疫组化和免疫组化。使用SDS [十二烷基硫酸钠]聚丙烯酰胺凝胶电泳结合免疫印迹和脑GFAP的多克隆抗血清，显示外周GFAP免疫反应性存在于与大鼠脑GFAP相同的MW为49 kd [千道尔顿]的多肽中。这种GFAP反应性可以在PNS中的各种神经中的雪旺细胞中以及在感觉神经节和交感神经节中的一些卫星细胞中以及肠神经胶质中以化学方法检测到。GFAP丝在雪旺氏细胞中的分布模式表明，在所调查的神经中，它们可能由大多数或所有非髓鞘形成的雪旺氏细胞表达，但不是由髓鞘形成的雪旺氏细胞表达。在免疫组织化学和免疫印迹研究中使用GFAP的单克隆抗体（抗GFAP-3），在大多数外周神经胶质细胞中发现的GFAP与星形胶质细胞不相同，因为它缺乏由该单克隆抗体定义的抗原决定簇，其存在于星形胶质细胞中。这一发现的一个例外是在肌间神经丛中发现的，其中使用单克隆抗体在一些但不是全部的肠神经胶质中发现了化学检测的GFAP。GFA多肽可能是一个异质组，其共享一些共同的决定簇和共同的MW，并且在CNS和PNS的神经胶质中显示出广泛和复杂的分布。
Glial fibrillary acidic protein [GFAP] is firmly established as the predominant component of astrocyte intermediate filaments. It has also been detected immunohistochemically in the glial cells of the enteric nervous system and some Schwann cells in the PNS [peripheral nervous system]. The molecular identity of this GFAP immunoreactivity in the PNS has so far not been investigated. This study compares GFAP in the CNS and PNS of adult rats both immunochemically and immunohisto chemically. Using SDS [sodium dodecyl sulfate] polyacrylamide gel electrophoresis combined with immunoblotting, and a polyclonal antiserum to brain GFAP, the peripheral GFAP immunoreactivity was shown to reside in a polypeptide with a MW of 49 kd [kiloDalton]which is identical to that of rat brain GFAP. This GFAP reactivity can be detected immunohistochemically in Schwann cells in a wide variety of nerves in the PNS and in some satellite cells in both sensory and sympathetic ganglia, in addition to enteric glia. The pattern of distribution of GFAP filaments in Schwann cells suggests that, in the nerves surveyed, they may be expressed by most or all non-myelin forming Schwann cells but not by myelin-forming Schwann cells. Using a monoclonal antibody to GFAP (anti-GFAP-3) in both immunohistochemical and immunoblotting studies, the GFAP found in most peripheral glia is not identical to that of astrocytes since it lacks an antigenic determinant, defined by this monoclonal antibody, which is present in astrocytes. An exception to this finding is seen in the myenteric plexuses where immunohistochemically detectable GFAP is found in some, but not all, of the enteric glia, using the monoclonal antibody. GFA polypeptides may be a heterogeneous group, that share some common determinants and a common MW, and show a widespread and complex distribution in the glia of both the CNS and PNS.