Excitatory effect of substance P in parasympathetic ganglia of cat urinary bladder.
Excitatory effect of substance P in parasympathetic ganglia of cat urinary bladder.
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P物质对猫膀胱副交感神经节的兴奋作用。
DOI：
10.1152/ajpregu.1989.257.6.r1450
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
deGroat,WC
中科院分区：
文献类型：
--
作者：
Kawatani,M;Whitney,T;Booth,AM;deGroat,WC
经动脉给药P物质（0.1-50微克/千克）后，猫的膀胱产生缓慢持续的膀胱收缩，膀胱神经节后神经不同步放电，并促进烟碱在膀胱神经节的传递。体外膀胱神经节细胞内记录显示P物质使神经节细胞去极化，并引发动作电位爆发（最大频率6 ~ 7 Hz）。γ -氨基丁酸（2-20微克/千克）和P物质拮抗剂[D-Arg1，D-Pro2,D-Trp7.9，Leu11] P物质（0.5-20微克/千克）可阻断原位P物质的神经节兴奋作用，但阿托品（10-100微克/千克）、六甲铵（0.5-2毫克/千克）、去甲肾上腺素（2-20微克/千克）或亮氨酸脑啡肽（0.5-20微克/千克）不改变原位P物质的兴奋作用。P物质引起的膀胱收缩不被阿托品、六甲铵或[D-Arg1，D-Pro2,D-Trp7.9，Leu11] P物质（0.1-10微克/千克）阻断，但被另一种P物质拮抗剂[D-Pro2，D-Phe7，D-Trp9] P物质阻断。这些数据表明P物质对膀胱副交感神经节神经元和膀胱平滑肌细胞具有直接的突触后兴奋作用。P物质拮抗剂对这两个部位兴奋反应的不同影响表明，这些反应是由不同类型的速激肽受体介导的。
Intra-arterial administration of substance P (0.1-50 micrograms/kg) to the urinary bladder of the cat produced slow-onset and sustained bladder contractions, asynchronous firing of bladder postganglionic nerves, and facilitation of nicotinic transmission in bladder ganglia. Intracellular recording from bladder ganglia in vitro revealed that substance P depolarized ganglion cells and initiated burst of action potentials (maximal frequency 6-7 Hz). The ganglionic excitatory effect of substance P in situ was blocked by gamma-aminobutyric acid (2-20 micrograms/kg) and the substance P antagonist [D-Arg1,D-Pro2,D-Trp7.9,Leu11]substance P (0.5-20 micrograms/kg) but was not altered by atropine (10-100 micrograms/kg), hexamethonium (0.5-2 mg/kg), norepinephrine (2-20 micrograms/kg), or leucine enkephalin (0.5-20 micrograms/kg). The bladder contractions elicited by substance P were not blocked by atropine, hexamethonium, or [D-Arg1,D-Pro2,D-Trp7.9,Leu11]substance P (0.1-10 micrograms/kg) but were blocked by another substance P antagonist, [D-Pro2,D-Phe7,D-Trp9]substance P. These data indicate that substance P has a direct postsynaptic excitatory effect on neurons in the vesical parasympathetic ganglia and on bladder smooth muscle cells. The differential effects of substance P antagonists on the excitatory responses at these two sites indicate the responses were mediated by different types of tachykinin receptors.