Innate immune receptor expression in peri-implant tissues of patients with different susceptibility to periodontal diseases.
Innate immune receptor expression in peri-implant tissues of patients with different susceptibility to periodontal diseases.
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DOI：
10.1902/jop.2012.120061
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发表时间：
2013-01
影响因子：
4.3
通讯作者：
Theofilos Koutouzis;Dominick Catania;K. Neiva;S. Wallet
中科院分区：
文献类型：
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作者：
Theofilos Koutouzis;Dominick Catania;K. Neiva;S. Wallet
背景虽然炎症介导牙周病的发病机制，但先天免疫反应对种植治疗的影响尚未得到评估。先天免疫受体，包括 Toll 样受体 (TLR) 和高级糖化终产物受体 (RAGE)，在发炎的牙龈内表达上调，并负责引发有害的宿主反应。本研究的目的是比较种植体治疗前后易患牙周炎的参与者和不易患牙周炎的参与者牙龈组织中TLR2、TLR4和RAGE的表达。方法 牙周健康的参与者接受了非牙周缺牙症的种植治疗。易患牙周炎的参与者在种植治疗前被诊断患有慢性牙周炎。在种植体安装前和治疗后 2 个月从种植体周围粘膜收集牙龈活检。使用组织学、实时 PCR 和蛋白质印迹来评估炎症浸润、TLR2、TLR4 和 RAGE 表达水平。结果 在种植治疗前，与牙周组织健康的参与者相比，易患牙周炎的参与者的牙龈组织中检测到 RAGE 水平升高，而 TLR2 或 TLR4 的表达没有检测到差异。种植治疗后，易患牙周炎的参与者的活组织检查中，RAGE 和 TLR4 水平上调，同时 TLR2 水平下调。在牙周组织健康的参与者的活检中，RAGE 和 TLR4 的水平保持不变，而 TLR2 的水平显着上调。从组织学角度来看，易患牙周炎的参与者的种植后活检显示出较高水平的炎症浸润。结论 易患牙周炎的参与者在种植体治疗后发现潜在炎症水平升高。
BACKGROUND Although inflammation mediates the pathogenesis of periodontal diseases, the effects of innate immune responses on implant therapies have not been evaluated. Innate immune receptors, including toll-like-receptors (TLRs) and the receptor for advanced glycated end-products (RAGE), are upregulated within inflamed gingiva and are responsible for initiation of detrimental host responses. The aim of this study is to compare the expression of TLR2, TLR4, and RAGE in gingival tissues from participants susceptible to periodontitis and participants not susceptible to periodontitis before and after implant therapy. METHODS Periodontally healthy participants received implant therapy for non-periodontal edentulism. Participants susceptible to periodontitis were diagnosed with chronic periodontitis prior to implant therapy. Gingival biopsies were collected from edentulous ridges before implant installation and from peri-implant mucosa 2 months after treatment. Histology, real-time PCR, and Western blot were used to evaluate levels of inflammatory infiltrate, TLR2, TLR4, and RAGE expression. RESULTS Before implant therapy, elevated levels of RAGE were detected in gingival tissues from participants susceptible to periodontitis when compared to those from participants with healthy periodontiums, whereas no differences in the expression of TLR2 or TLR4 were detected. After implant therapy, there was an upregulation of RAGE and TLR4 levels that coincided with a downregulation of TLR2 levels in biopsies from participants susceptible to periodontitis. Levels of RAGE and TLR4 remained unchanged in biopsies from participants with healthy periodontiums, whereas TLR2 levels were significantly upregulated. Histologically, post-implant biopsies from participants susceptible to periodontitis displayed higher levels of inflammatory infiltrate. CONCLUSION Elevated levels of inflammatory potential were found after implant therapy in participants susceptible to periodontitis.