Self-Assembly of Thermoresponsive Recombinant Silk-Elastinlike Nanogels
Self-Assembly of Thermoresponsive Recombinant Silk-Elastinlike Nanogels
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DOI：
10.1002/mabi.201700192
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发表时间：
2018-01-01
影响因子：
4.6
通讯作者：
Ghandehari, Hamidreza
中科院分区：
文献类型：
--
作者：
Isaacson, Kyle J.;Jensen, Mark Martin;Ghandehari, Hamidreza
重组丝弹性蛋白样蛋白聚合物 (SELP) 将人类原弹性蛋白的生物相容性和热响应性与丝的强度结合在一起。直接控制这些单分散聚合物的结构可以实现结构与功能的精确关联。这项工作描述了第一个 SELP 纳米凝胶的制造及其物理化学性质和热响应性的评估。由于物理交联的 β-折叠丝片段，稀浓度 SELP 的自组装导致纳米凝胶比胶束具有更高的稳定性。纳米凝胶通过尺寸变化和聚集来响应热刺激。改变聚合物主链中丝与弹性蛋白的比例和序列会导致临界凝胶形成浓度、稳定性、聚集、尺寸收缩温度和热可逆性的改变。纳米凝胶可以隔离疏水性化合物，并在生物活性剂的递送方面显示出前景。
Recombinant silk-elastinlike protein polymers (SELPs) combine the biocompatibility and thermoresponsiveness of human tropoelastin with the strength of silk. Direct control over structure of these monodisperse polymers allows for precise correlation of structure with function. This work describes the fabrication of the first SELP nanogels and evaluation of their physicochemical properties and thermoresponsiveness. Self-assembly of dilute concentrations of SELPs results in nanogels with enhanced stability over micelles due to physically crosslinked beta-sheet silk segments. The nanogels respond to thermal stimuli via size changes and aggregation. Modifying the ratio and sequence of silk to elastin in the polymer backbone results in alterations in critical gel formation concentration, stability, aggregation, size contraction temperature, and thermal reversibility. The nanogels sequester hydrophobic compounds and show promise in delivery of bioactive agents.