Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal
Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal
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DOI：
10.1095/biolreprod.102.013276
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发表时间：
2003-06-01
影响因子：
3.6
通讯作者：
Sawada, H
中科院分区：
文献类型：
--
作者：
Ohmura, M;Ogawa, T;Sawada, H
最近的研究表明，在睾丸损伤后给予gnrh类似物可以刺激大鼠的精子再生。虽然这一现象的机制尚未阐明，但有人提出支持细胞产生的干细胞因子（stem cell factor， SCF）介导gnrh类似物对精原细胞增殖和/或存活的影响。本研究采用精原细胞移植和激光共聚焦显微观察相结合的新方法，定量评价了精原细胞的增殖，并探讨了SCF是否介导gnrh -类似物对精原细胞增殖的影响。在第一个实验中，以野生型小鼠为受者进行精原细胞移植，在移植后4周和5周，给予gnrh激动剂leuprorelin的实验组小鼠在每个生精集落中每100个Sertoli细胞中供体精原细胞的数量明显高于对照组。在第二个实验中，使用缺乏膜结合形式SCIF表达的Steel/Steel(dickie) （SI/SId）突变小鼠作为受体。从第一个实验中可以看出，leuprorelin处理组未分化精原细胞的数量明显高于对照组。由于未分化的精原细胞不表达SCF受体，本研究清楚地表明，无论是膜结合形式还是分泌形式的SCF都不参与gnrh -类似物对精原细胞增殖和/或存活的影响机制。
Recent studies have demonstrated that GnRH-analogues can stimulate regeneration of spermatogenesis of rats when administered after testicular damages. Although the mechanism of this phenomenon has not been elucidated yet, stem cell factor (SCF) produced by Sertoli cells was proposed to mediate the effects of GnRH-analogues on spermatogonial proliferation and/or survival. In the present study, we quantitatively evaluated the proliferation of spermatogonia and addressed whether SCF mediates the effect of GnRH-analogue on spermatogonial proliferation, using a novel approach combining spermatogonial transplantation and laser confocal microscopic observation. In the first experiment, using wild-type mice as recipients for spermatogonial transplantation, the number of donor spermatogonia per 100 Sertoli cells in each spermatogenic colony was significantly higher in the experimental group of mice treated with leuprorelin, a GnRH-agonist, than that of the control group at 4 and 5 wk after transplantation. In the second experiment, Steel/Steel(dickie) (SI/SId) mutant mice, which lack expression of membrane bound form SCIF, were used as recipients. As seen in the first experiment, the number of undifferentiated spermatogonia was significantly higher in leuprorelin-treated than in the control group. Since undifferentiated spermatogonia do not express the receptor of SCF, the present study clearly demonstrates that neither membrane-bound nor secreted forms of SCF are involved in the mechanism of GnRH-analogue's effect on spermatogonial proliferation and/or survival.