A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex
A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex
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DOI：
10.1074/jbc.m509128200
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发表时间：
2005-12-16
影响因子：
4.8
通讯作者：
Conaway, JW
中科院分区：
文献类型：
--
作者：
Jin, JY;Cai, Y;Conaway, JW
哺乳动物Tip49a和Tip49b蛋白属于进化保守的AAA(+)ATPase家族。在酿酒酵母中，Tip49a和Tip49b的同源基因分别称为Rvb1和Rvb2，是两个不同的依赖于ATP的染色质重塑复合体SWR1和INO80的亚基。我们最近证明了哺乳动物的Tip49a和Tip49b蛋白是染色质重塑复合体的组成部分，与酿酒酵母SWR1复合体具有惊人的相似之处(Cai，Y.，jin，J.，Florens，L.，Swanson，S.K.，Kusch，T.，Li，B.，Workman，J.L.，沃什伯恩，M.P.，Conaway，R.C.和Conaway，J.W.(2005)J.Biol。化学。280、13665-13670)。在本报告中，我们发现了一个新的哺乳动物Tip49a-和Tip49b-包含依赖于ATP的染色质重塑复合体，它包括酿酒酵母INO80染色质重塑复合体15个亚基中的8个的同源基因，以及至少5个人类INO80(HINO80)复合体特有的额外亚基。最后，我们证明，与酵母INO80复合体类似，hINO80复合体具有DNA和核小体激活的ATPase活性，并催化依赖于ATP的核小体滑动。
The mammalian Tip49a and Tip49b proteins belong to an evolutionarily conserved family of AAA(+) ATPases. In Saccharomyces cerevisiae, orthologs of Tip49a and Tip49b, called Rvb1 and Rvb2, respectively, are subunits of two distinct ATP-dependent chromatin remodeling complexes, SWR1 and INO80. We recently demonstrated that the mammalian Tip49a and Tip49b proteins are integral subunits of a chromatin remodeling complex bearing striking similarities to the S. cerevisiae SWR1 complex (Cai, Y., Jin, J., Florens, L., Swanson, S. K., Kusch, T., Li, B., Workman, J. L., Washburn, M. P., Conaway, R. C., and Conaway, J. W. ( 2005) J. Biol. Chem. 280, 13665-13670). In this report, we identify a new mammalian Tip49a- and Tip49b- containing ATP-dependent chromatin remodeling complex, which includes orthologs of 8 of the 15 subunits of the S. cerevisiae INO80 chromatin remodeling complex as well as at least five additional subunits unique to the human INO80 (hINO80) complex. Finally, we demonstrate that, similar to the yeast INO80 complex, the hINO80 complex exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.