The NSm proteins of Rift Valley fever virus are dispensable for maturation, replication and infection
The NSm proteins of Rift Valley fever virus are dispensable for maturation, replication and infection
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DOI：
10.1016/j.virol.2006.09.035
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发表时间：
2007-03-15
期刊：
影响因子：
3.7
通讯作者：
Nichol, Stuart T.
中科院分区：
文献类型：
--
作者：
Gerrard, Sonja R.;Bird, Brian H.;Nichol, Stuart T.
裂谷热病毒属于布尼亚病毒科分段负链RNA病毒，在撒哈拉以南非洲引起蚊媒疾病。我们报告了针对埃及强毒株ZH501的T7 RNA聚合酶驱动的基于质粒的遗传系统的开发。我们已经使用该系统来拯救一种病毒，该病毒在基因组M片段上具有387个核苷酸的缺失，该缺失消除了两种称为NSm的非结构蛋白的编码区。该病毒Delta NSm rZH501在结构蛋白的表达和在培养的哺乳动物细胞中的生长方面与亲本ZH501毒株没有区别。爱思唯尔公司出版
Rift Valley fever (RVF) virus belongs to the Bunyaviridae family of segmented negative-strand RNA viruses and causes mosquito-borne disease in sub-Saharan Africa. We report the development of a T7 RNA polymerase-driven plasmid-based genetic system for the virulent Egyptian isolate, ZH501. We have used this system to rescue a virus that has a 387 nucleotide deletion on the genomic M segment that eliminates the coding region for two non-structural proteins known as NSm. This virus, Delta NSm rZH501, is indistinguishable from the parental ZH501 strain with respect to expression of structural proteins and growth in cultured mammalian cells. Published by Elsevier Inc.