Basic Biological Sciences Isolation and Growth of Human Periodontal Ligament Cells in vitro
Basic Biological Sciences Isolation and Growth of Human Periodontal Ligament Cells in vitro
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基础生物科学 人牙周膜细胞的体外分离和生长
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
J. Daniel
中科院分区：
文献类型：
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作者：
B. Ragnarsson;G. Carr;J. Daniel
将人单个牙齿的牙周韧带从牙根表面剥离并接种到培养皿中。在每种情况下，获得两个细胞群：成纤维细胞和上皮细胞。将这两个群体分离并在培养物中生长。通过角蛋白（一种专门的上皮细胞蛋白）染色证实了类上皮细胞的上皮性质。
The periodontal ligaments from single human teeth were dissected free of the root surface and plated into culture dishes. In each case two populations of cells were obtained: fibroblasts and epithelial cells. These two populations were separated and grown in culture. The epithelial nature of the epithelioid-appearing cells was confirmed by staining for keratin, exclusively an epithelial cell protein.