An Olfactory Shift Is Associated with Male Perfume Differentiation and Species Divergence in Orchid Bees
An Olfactory Shift Is Associated with Male Perfume Differentiation and Species Divergence in Orchid Bees
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DOI：
10.1016/j.cub.2008.10.049
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发表时间：
2008-12-09
期刊：
影响因子：
9.2
通讯作者：
Lunau, Klaus
中科院分区：
文献类型：
--
作者：
Eltz, Thomas;Zimmermann, Yvonne;Lunau, Klaus
突变变化可能是昆虫信息素通信系统多样化的基础，昆虫信息素通信系统通常处于稳定选择下，有利于信号和信号感知的高特异性[1-4]。在兰花蜂（Euglossini）中，雄性信号的产生依赖于嗅觉：雄性从环境中收集复杂的挥发物（香水）混合物[5,6]，这些挥发物随后在交配地点[7]作为信息素类似物释放出来。本文分析了墨西哥两种不同下颌牙齿数量的雄性Euglossa cf. viridissima对香水成分的行为和触角反应。三叉雄性收集2-羟基-6-壬基-1,3-二烯基苯甲醛（HNDB）作为其香水的主要成分。在双齿雄性中，混合物大致相似，但缺乏HNDB。种群遗传分析表明，三齿和双齿雄性属于两个生殖分离的谱系。触角电图测试（EAG和GC-EAD）显示，双齿雄蛛对HNDB的触角反应明显低于三叉雄蛛，首次揭示了近亲物种从其栖息地获取不同化合物的机制。两个兄弟物种雄性在香水感知和收集方面的成分特异性差异与嗅觉驱动的信号香水进化模式一致。然而，雌性对分化信号的反应尚不清楚。
Saltational changes may underlie the diversification of pheromone communication systems in insects, which are normally under stabilizing selection favoring high specificity in signals and signal perception [1-4]. In orchid bees (Euglossini), the production of male signals depends on the sense of smell: males collect complex blends of volatiles (perfumes) from their environment [5,6], which are later emitted as pheromone analogs at mating sites [7]. We analyzed the behavioral and antennal response to perfume components in two male morphotypes of Euglossa cf. viridissima from Mexico, which differ in the number of mandibular teeth. Tridentate males collected 2-hydroxy-6-nona-1,3-dienyl-benzaldehyde (HNDB) as the dominant component of their perfume. In bidentate males, blends were broadly similar but lacked HNDB. Population genetic analysis revealed that tri- and bidentate males belong to two reproductively isolated lineages. Electroantennogram tests (EAG and GC-EAD) showed substantially lower antennal responses to HNDB in bidentate versus tridentate males, revealing for the first time a mechanism by which closely related species acquire different chemical compounds from their habitat. The component-specific differences in perfume perception and collection in males of two sibling species are in agreement with a saltational, olfaction-driven mode of signal perfume evolution. However, the response of females to the diverged signals remains unknown.