HOMEOTIC TRANSFORMATION OF CERVICAL-VERTEBRAE IN HOXA-4 MUTANT MICE
HOMEOTIC TRANSFORMATION OF CERVICAL-VERTEBRAE IN HOXA-4 MUTANT MICE
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DOI：
10.1073/pnas.91.26.12644
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发表时间：
1994-12-20
影响因子：
11.1
通讯作者：
BEHRINGER, RR
中科院分区：
文献类型：
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作者：
HORAN, GSB;WU, K;BEHRINGER, RR
Hoxa-4（以前称为Hox-1.4）是一种小鼠同源框基因，在胚胎发育期间在推测的后脑和脊髓，前椎骨和其他组织中表达。为了了解Hoxa-4在发育过程中的作用，我们产生了Hoxa-4突变小鼠。纯合突变体是可行的且可繁殖的。对新生儿骨骼的分析显示，第七颈椎上肋骨的发育具有可变的延伸率和表现力。还观察到胸骨形态发生的低频率改变。此外，我们分析了过表达Hoxa-4的转基因小鼠的骨骼，发现通常在第七颈椎上发育的小肋骨原基的形成受到抑制。对成年同型合子突变体骨骼的分析显示，通常与第二颈椎相关的背突也出现在第三颈椎上。这些结果表明，Hoxa-4在沿着前后轴提供位置信息以指定第三和第七颈椎的身份方面发挥作用。
Hoxa-4 (previously known as Hox-1.4) is a mouse homeobox-containing gene that is expressed in the presumptive hindbrain and spinal cord, prevertebrae, and other tissues during embryogenesis. To understand the role of Hoxa-4 during development, we generated Hoxa-4 mutant mice. Homozygous mutants were viable and fertile. Analysis of neonatal skeletons revealed the development of ribs on the seventh cervical vertebra at variable penetrance and expressivity. A low frequency of alterations in sternal morphogenesis was also observed. In addition, we analyzed the skeletons of transgenic mice that overexpress Hoxa-4 and found that the formation of the small rib anlagen that often develop on the seventh cervical vertebra was suppressed. Analysis of adult homozyogus mutant skeletons revealed that the dorsal process normally associated with the second cervical vertebra was also found on the third cervical vertebra. These results demonstrate that Hoxa-4 plays a role in conferring positional information along the anteroposterior axis to specify the identity of the third and the seventh cervical vertebrae.