Energy-dependent transport of nickel by Clostridium pasteurianum.
Energy-dependent transport of nickel by Clostridium pasteurianum.
复制标题
巴氏梭菌对镍的能量依赖性运输。
DOI：
10.1128/jb.170.1.234-238.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
Drake,HL
中科院分区：
文献类型：
--
作者：
Bryson,MF;Drake,HL
采用63 NiCl 2和微滤法研究了巴氏梭菌对镍的转运机制。镍运输是能量依赖性的，需要葡萄糖或蔗糖;木糖和O-甲基葡萄糖不支持生长，butyrogenesis，或运输。运输是最佳的pH值为7和37摄氏度，和早期稳定期细胞具有最高的倾向镍运输。镍转运的表观Km和Vmax分别约为85 μ M Ni和1，400 pmol Ni转运/min/mg（干重）细胞。基于金属特异性，镍似乎主要通过镁转运蛋白转运，尽管也可能涉及替代性镍转运蛋白。ATP酶抑制剂（N，N '-二环己基碳二亚胺、氯化三丁基锡、7-氯-4-硝基苯-2-氧杂-1，3-二唑和槲皮素）、质子载体（羰基氰间氯苯腙、2，4-二硝基苯酚和短杆菌肽D）、金属离子载体（缬氨霉素、莫能菌素和尼日利亚菌素）、苄基紫精、一氧化碳和氧抑制镍转运。镍的运输是间接耦合到butyrogenesis和依赖于能量状态的细胞。
The mechanism of nickel transport by Clostridium pasteurianum was investigated by using 63NiCl2 and a microfiltration transport assay. Nickel transport was energy dependent, requiring either glucose or sucrose; xylose and o-methyl glucose did not support growth, butyrogenesis, or transport. Transport was optimum at pH 7 and 37 degrees C, and early-stationary-phase cells had the highest propensity for nickel transport. The apparent Km and Vmax for nickel transport approximated 85 microM Ni and 1,400 pmol of Ni transported per min per mg (dry weight) of cells, respectively. On the basis of metal specificity, nickel appears to be transported primarily by a magnesium transporter, although an alternative nickel transporter may also be involved. ATPase inhibitors (N,N'-dicyclohexylcarbodiimide, tributyltin chloride, 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, and quercetin), protonophores (carbonyl cyanide m-chlorophenylhydrazone, 2,4-dinitrophenol, and gramicidin D), metal ionophores (valinomycin, monensin, and nigericin), benzyl viologen, carbon monoxide, and oxygen inhibited nickel transport. Nickel transport was coupled indirectly to butyrogenesis and was dependent on the energy state of the cell.