Mutations at key pore-lining positions differentiate the water permeability of fish lens aquaporin from other vertebrates.
Mutations at key pore-lining positions differentiate the water permeability of fish lens aquaporin from other vertebrates.
复制标题
关键孔衬位置的突变使鱼晶状体水通道蛋白的透水性与其他脊椎动物不同。
DOI：
10.1016/j.febslet.2010.10.058
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发表时间：
2010
期刊：
影响因子：
3.5
通讯作者：
Calvanese L
中科院分区：
文献类型：
--
作者：
Calvanese L
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Aquaporin-0 (AQP0) is the major integral membrane protein of lens fiber cell and helps to maintain lens transparency by mediating inter-cell adhesion. To shed light on the unexpected higher water transport efficiency of killifish AQP0 as compared to mammalian orthologues, we performed a comparative analysis of all available AQP0 sequences and built 3D-models for representatives of different vertebrate classes. The analysis shows that air-living organisms evolved specific mutations at pore-lining positions to modulate the AQP0 water transport efficiency while maintaining the correct tertiary/quaternary arrangement to allow the formation of “thin junctions” between lens fiber cells. We conclude that the low permeability of mammalian AQP0 is required not to promote cell adhesion, but to modulate the water balance in a dry environment.