Proteomic profiling of mitochondria: what does it tell us about the ageing brain?
Proteomic profiling of mitochondria: what does it tell us about the ageing brain?
复制标题
线粒体的蛋白质组学分析：它告诉我们有关衰老大脑的什么？
DOI：
10.18632/aging.101131
复制
发表时间：
2016-12-13
期刊：
影响因子：
--
通讯作者：
Chakrabarti L
中科院分区：
文献类型：
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作者：
Ingram T;Chakrabarti L
线粒体功能障碍在许多神经退行性疾病和年龄相关疾病中是明显的。它还与细胞衰老有关，但我们目前对线粒体变化的了解还不清楚。功能研究已经取得了一些进展，报告呼吸减少，动态结构修改和膜电位的损失，但这些发现之间存在矛盾。蛋白质组学分析，连同功能研究，是必需的，以配置文件的线粒体的变化，发生随着年龄的增长，可以有助于解开衰老表型的复杂性。改进的蛋白质分离技术的出现，与质谱分析相结合，允许识别能量代谢，抗氧化剂，融合和裂变机制，伴侣蛋白，膜蛋白和生物合成途径中的年龄和细胞类型特异性线粒体变化。在这里，我们确定和审查最近的数据从啮齿动物大脑的线粒体分析。预计从了解与年龄相关的大脑线粒体变化中获得的知识应该会导致正常衰老和与年龄相关的疾病进展的生物标志物的改善。
Mitochondrial dysfunction is evident in numerous neurodegenerative and age-related disorders. It has also been linked to cellular ageing, however our current understanding of the mitochondrial changes that occur are unclear. Functional studies have made some progress reporting reduced respiration, dynamic structural modifications and loss of membrane potential, though there are conflicts within these findings. Proteomic analyses, together with functional studies, are required in order to profile the mitochondrial changes that occur with age and can contribute to unravelling the complexity of the ageing phenotype. The emergence of improved protein separation techniques, combined with mass spectrometry analyses has allowed the identification of age and cell-type specific mitochondrial changes in energy metabolism, antioxidants, fusion and fission machinery, chaperones, membrane proteins and biosynthesis pathways. Here, we identify and review recent data from the analyses of mitochondria from rodent brains. It is expected that knowledge gained from understanding age-related mitochondrial changes of the brain should lead to improved biomarkers of normal ageing and also age-related disease progression.