Application of the Hybrid Capture 2 assay to squamous cell carcinomas of the head and neck A convenient liquid-phase approach for the reliable determination of human papillomavirus status
Application of the Hybrid Capture 2 assay to squamous cell carcinomas of the head and neck A convenient liquid-phase approach for the reliable determination of human papillomavirus status
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DOI：
10.1002/cncy.20175
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发表时间：
2012-02-25
影响因子：
3.4
通讯作者：
Westra, William H.
中科院分区：
文献类型：
--
作者：
Bishop, Justin A.;Maleki, Zahra;Westra, William H.
背景：越来越多的头颈部鳞状细胞癌（HNSCC）是由人乳头瘤病毒（HPV）引起的。鉴于HPV相关HNSCCs独特的自然史和预后，常规HPV检测正被纳入诊断方案。因此，对最佳检测策略的需求不断增加，这种策略必须敏感和特异，可转移到诊断实验室，跨实验室标准化，具有成本效益，并可广泛应用于包括细胞学制剂在内的各种标本类型。方法：从手术切除的HNSCCs中获得细胞学制剂（细针抽吸剂[FNAs]和刷子），并使用Hybrid Capture 2检测评估高危HPV的存在。采用HPV原位杂交和p16免疫组织化学对相应组织切片进行HPV分析。在免疫组化和原位杂交结果不一致的病例中，通过实时聚合酶链反应检测E7表达来确定HPV状态。比较组织中HPV的状态和相应的细胞学样本。结果：基于组织切片的基准HPV检测，14例HNSCCs为HPV阳性，10例为HPV阴性。所有相应的细胞学制剂使用杂交捕获2试验正确分类。结论：杂交捕获2策略，已经广泛用于检测宫颈刷中的高危HPV，很容易转移到HNSCCs。细胞学准备的一致性准确性表明其在淋巴结转移患者的FNAs中的潜在应用，并且可能消除仅为HPV检测目的而获取组织的需要。癌症（Cancer Cytopathol） 2012；120:年龄在18岁至25岁之间。(C) 2011年美国癌症协会。
BACKGROUND: A growing proportion of head and neck squamous cell carcinoma (HNSCC) is caused by the human papillomavirus (HPV). In light of the unique natural history and prognosis of HPV-related HNSCCs, routine HPV testing is being incorporated into diagnostic protocols. Accordingly, there is an escalating demand for an optimal detection strategy that is sensitive and specific, transferrable to the diagnostic laboratory, standardized across laboratories, cost-effective, and amenable to broad application across specimen types including cytologic preparations. METHODS: Cytologic preparations (fine-needle aspirates [FNAs] and brushes) were obtained from surgically resected HNSCCs and evaluated for the presence of high-risk HPV using the Hybrid Capture 2 assay. HPV analysis was also performed on the corresponding tissue sections using HPV in situ hybridization and p16 immunohistochemistry. In cases in which the immunohistochemical and in situ hybridization results were discordant, HPV status was determined by real-time polymerase chain reaction detection of E7 expression. HPV status in the tissues and corresponding cytologic samples was compared. RESULTS: Based on benchmark HPV testing of the tissue sections, 14 HNSCCs were classified as HPV positive and 10 as HPV negative. All corresponding cytologic preparations were correctly classified using the Hybrid Capture 2 assay. CONCLUSIONS: The Hybrid Capture 2 strategy, already widely used for the detection of high-risk HPV in cervical brushes, is readily transferrable to HNSCCs. Consistent accuracy in cytologic preparation suggests its potential application in FNAs from patients who present with lymph node metastases, and may eliminate the need to obtain tissue solely for the purpose of HPV testing. Cancer (Cancer Cytopathol) 2012; 120: 18-25. (C) 2011 American Cancer Society.