A chemical approach to mapping nucleosomes at base pair resolution in yeast.
A chemical approach to mapping nucleosomes at base pair resolution in yeast.
复制标题
DOI：
10.1016/b978-0-12-391938-0.00014-8
复制
发表时间：
2012
影响因子：
--
通讯作者：
Widom, Jonathan
中科院分区：
文献类型：
--
作者：
Brogaard, Kristin R.;Xi, Liqun;Wang, Ji-Ping;Widom, Jonathan
大多数真核DNA存在于DNA-蛋白质复合物中，称为核小体。核小体沿着基因组的确切位置在染色体功能和基因调控中起着关键作用。然而，目前的核小体定位方法不能提供必要的准确性来确定精确的核小体位置。在这里，我们描述了一种新的实验方法，直接映射核小体中心的位置在体内全基因组在单碱基对分辨率。
Most eukaryotic DNA exists in DNA–protein complexes known as nucleosomes. The exact locations of nucleosomes along the genome play a critical role in chromosome functions and gene regulation. However, the current methods for nucleosome mapping do not provide the necessary accuracy to identify the precise nucleosome locations. Here we describe a new experimental approach that directly maps nucleosome center locations in vivo genome-wide at single base pair resolution.