THE REGULATION OF GRANULOSA-CELL PROOPIOMELANOCORTIN MESSENGER-RIBONUCLEIC-ACID BY ANDROGENS AND GONADOTROPINS
THE REGULATION OF GRANULOSA-CELL PROOPIOMELANOCORTIN MESSENGER-RIBONUCLEIC-ACID BY ANDROGENS AND GONADOTROPINS
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DOI：
10.1210/endo-119-5-2082
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发表时间：
1986-11-01
期刊：
影响因子：
4.8
通讯作者：
KOOS, RD
中科院分区：
文献类型：
--
作者：
MELNER, MH;YOUNG, SL;KOOS, RD
我们以前已经证明了大鼠颗粒细胞中存在前阿片黑素皮质素(POMC)信使RNA(MRNA)。这项研究考察了激素对颗粒细胞POMC mRNA水平的独特的、组织特异性的调节，这些激素对这些细胞具有既定的调节作用。对未成熟的大鼠卵巢RNA的Northern杂交分析表明，存在一个长度约为900个碱基对的单一mRNA。用PMSG刺激48h后，卵巢POMC基因的表达水平以每微克总RNA计增加了约6倍。分离未成熟PMSG诱导的大鼠颗粒细胞，在无血清条件下培养，观察其对POMC基因表达水平的影响。用黄体生成素(100 ng/ml)、雄烯二酮(10-7M)或黄体生成素+雄烯二酮处理细胞48h，POMC基因表达水平升高。单用黄体生成素可使POMC基因表达增加5倍，雄烯二酮可使其增加10倍；联合用药可使其增加47倍。对非芳香化雄激素双氢睾酮(DHT)的作用也进行了评估。DHT(10-7M)处理48h后，POMC基因表达水平增加40倍。体内检测未成熟雌鼠卵巢POMC mRNA的实验证实了体外实验结果。皮下注射DHT(500微克)的动物显示，当每微克总RNA表达时，卵巢POMC mRNA增加了5倍。这些研究提供了体外和体内证据，证明颗粒细胞POMC mRNA受到雄激素和促性腺激素的独特激素调节。
We have previously demonstrated the presence of proopiomelanocortin (POMC) messenger RNA (mRNA) in rat granulosa cells. This study examines the unique, tissue-specific regulation of granulosa cell POMC mRNA levels by hormones with established regulatory effects on these cells. Northern blot analysis of immature rat ovarian RNA revealed the presence of a single mRNA of approximately 900 base pairs in length. The levels of ovarian POMC mRNA increased approximately 6-fold 48 h after priming with PMSG when expressed per microgram of total RNA. Granulosa cells were isolated from immature PMSG-primed rats and cultured under serum-free conditions in the presence and absence of various hormones to determine their effect on POMC mRNA levels. Treatment of the cells for 48 h with LH (100 ng/ml), androstenedione (10-7 M), or LH plus androstenedione elevated POMC mRNA levels. LH alone elicited a 5-fold increase in POMC mRNA, and androstenedione elicited a 10-fold increase; the combination treatment led to a 47-fold increase. The effects of the nonaromatizable androgen dihydrotestosterone (DHT) were also evaluated. Treatment for 48 h with DHT (10-7 M) elicited a 40-fold increase in POMC mRNA levels. In vivo experiments measuring ovarian POMC mRNA from immature female rates corroborated the in vitro results. Animals injected sc with DHT (500 .mu.g) demonstrated 5-fold increases in ovarian POMC mRNA when expressed per microgram of total RNA. These studies provide both in vitro and in vivo evidence that granulosa cell POMC mRNA is under unique hormonal regulation by both androgens and gonadotropins.