Casein Kinase 1 Delta (CK1δ) Regulates Period Length of the Mouse Suprachiasmatic Circadian Clock In Vitro
Casein Kinase 1 Delta (CK1δ) Regulates Period Length of the Mouse Suprachiasmatic Circadian Clock In Vitro
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DOI：
10.1371/journal.pone.0010303
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发表时间：
2010-04-22
期刊：
影响因子：
3.7
通讯作者：
Weaver, David R.
中科院分区：
文献类型：
--
作者：
Etchegaray, Jean-Pierre;Yu, Elizabeth A.;Weaver, David R.
背景资料：酪蛋白激酶1 δ（CK 1 δ）在外周组织如肝脏和胚胎成纤维细胞中比其同源物酪蛋白激酶1 β（CK 1 β）在昼夜节律周期长度的调节中发挥更突出的作用。缺乏CK 1 δ的小鼠出生后不久死亡，因此无法评估CK 1 δ缺失对视交叉上核（SCN）中主昼夜节律振荡器控制的行为节律的影响。方法学/主要发现：在本研究中，从新生小鼠SCN外植体收集的mPER 2：：LUCIFERASE生物发光节律进行了监测。数据表明，SCN外植体从新生儿CK 1 δ缺陷小鼠振荡，但具有较长的昼夜节律周期比同窝对照。记录从新生儿SCN外植体CK 1 ε缺陷小鼠的周期长度的节奏没有从控制explors.Conclusions/意义：结果表明，CK 1 δ发挥更突出的作用比CK 1 β在主昼夜节律振荡器的24小时节律的维护。
Background: Casein kinase 1 delta (CK1 delta) plays a more prominent role in the regulation of circadian cycle length than its homologue casein kinase 1 epsilon (CK1 epsilon) in peripheral tissues such as liver and embryonic fibroblasts. Mice lacking CK1 delta die shortly after birth, so it has not been possible to assess the impact of loss of CK1 delta on behavioral rhythms controlled by the master circadian oscillator in the suprachiasmatic nuclei (SCN).Methodology/Principal Findings: In the present study, mPER2::LUCIFERASE bioluminescence rhythms were monitored from SCN explants collected from neonatal mice. The data demonstrate that SCN explants from neonatal CK1 delta-deficient mice oscillate, but with a longer circadian period than littermate controls. The cycle length of rhythms recorded from neonatal SCN explants of CK1 epsilon-deficient mice did not differ from control explants.Conclusions/Significance: The results indicate that CK1 delta plays a more prominent role than CK1 epsilon in the maintenance of 24-hour rhythms in the master circadian oscillator.