Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain
Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain
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DOI：
10.1016/j.molcatb.2009.06.012
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Sovova, Helena
中科院分区：
文献类型：
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作者：
Hlavsova, Klara;Zarevucka, Marie;Sovova, Helena
研究了白地霉Geotrichum candidum 4013在液体培养基中产生的两种脂肪酶（胞外型和细胞结合型）对黑加仑油水解的催化作用。分析反应产物的转化程度，从该转化程度评价酶活性，并将游离脂肪酸的组成与油底物的组成进行比较。还在SC-CO2中在反应之前和之后测量酶活性。通过水解从油中释放的酸的脂肪酸组合物表明来自白地霉4013的细胞结合酶和胞外酶的特异性。胞外脂肪酶对多不饱和脂肪酸的选择性较低，而细胞结合型脂肪酶对饱和脂肪酸具有选择性。用两种诱导酶实现的测试过程的对映选择性是高的（从43到242）。所有酶的活性显着增加后，他们暴露于SC-CO2。超临界CO2对酶的处理是提高酶促反应效率的一种简便易行的方法。(C)2009 Elsevier B. V.版权所有
Two types of lipases (extracellular and cell-bound) were produced by Geotrichum candidum 4013 in liquid medium and were used as biocatalysts in blackcurrant oil hydrolysis. Reaction products were analysed for the degree of conversion from which enzyme activity was evaluated, and the composition of free fatty acids was compared to the composition of oil substrate. The enzyme activity was measured also before and after the reaction in SC-CO2. The fatty acid composition of the acids liberated from oil by hydrolysis suggests a specificity of the cell-bound and extracellular enzymes from Geotrichum candidum 4013. The extracellular lipase displays low selectivity to the polyunsaturated fatty acids, and the cell-bound lipase possesses selectivity to the saturated fatty acids. Enantioselectivity of the tested processes achieved with both induced enzymes was high (from 43 to 242). The activity of all enzymes has markedly increased after their exposure to SC-CO2. The treatment of enzymes by SC-CO2 could be easy-to-use approaches to improve the efficiency of enzymatic reactions. (C) 2009 Elsevier B.V. All rights reserved