Biocompatible nanomembranes based on PEGylation of cross-linked self-assembled monolayers
Biocompatible nanomembranes based on PEGylation of cross-linked self-assembled monolayers
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DOI：
10.1021/cm3011875
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发表时间：
2011-06
影响因子：
8.6
通讯作者：
Nikolaus Meyerbröker;Ziyue Li;W. Eck;M. Zharnikov
中科院分区：
文献类型：
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作者：
Nikolaus Meyerbröker;Ziyue Li;W. Eck;M. Zharnikov
我们提出了基于4‘-硝基-1，1’-联苯酚-4-硫醇(NBPT)在Au(111)载体上的自组装单分子膜(SAM)制备独立的、生物相容的超薄薄膜。在第一步中，NBPT自组装膜通过暴露在低能电子下进行广泛的交联，得到了机械和热稳定的单分子膜。同时，NBPT分子的末端硝基被转化为反应胺部分，在第二步中，环氧官能化聚乙二醇(PEG)被偶联到反应胺部分上。根据椭偏仪和X射线光电子能谱的监测，只要偶联聚乙二醇层的厚度超过∼3 nm，所得到的组装就变得具有蛋白质抗性，并且在作为独立膜从底物上分离后不会失去这一性质(第三步)。在透射电子显微镜研究中使用这种膜作为载体，可以从根本上提高这类实验的分辨率和结构可靠性。
We present the fabrication of freestanding, biocompatible, and ultrathin membranes on the basis of self-assembled monolayers (SAMs) of 4′-nitro-1,1′-biphenol-4-thiol (NBPT) on an Au(111) support. In the first step, NBPT SAMs were extensively cross-linked by exposure to low-energy electrons, resulting in a mechanically and thermally stable monolayer. Simultaneously, the terminal nitro groups of the NBPT molecules were converted to reactive amine moieties to which, in the second step, epoxy-functionalized poly(ethylene glycols) (PEG) were coupled. As far as the thickness of the coupled PEG overlayer exceeded ∼3 nm, as monitored by ellipsometry and X-ray photoelectron spectroscopy, the resulting assembly became protein-resistant, without losing this property after separation from the substrate as a freestanding membrane (third step). The use of such membranes as supports in transmission electron microscopy studies may improve essentially the resolution and structural reliability of such experiments in their ...