Identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids
Identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids
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DOI：
10.1128/iai.71.10.6088-6094.2003
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发表时间：
2003-10-01
影响因子：
3.1
通讯作者：
Shinagawa, K
中科院分区：
文献类型：
--
作者：
Omoe, K;Hu, DL;Shinagawa, K
我们鉴定并鉴定了一种新的葡萄球菌肠毒素样毒素，命名为SER。Ser基因的核苷酸序列分析表明，Ser与seg基因关系最为密切。Ser基因产物SER在大肠杆菌中以重组蛋白的形式成功表达，重组SER(RSER)具有明显的T细胞刺激活性。用抗rSER抗体进行Western印迹分析，证实了携带SER的金黄色葡萄球菌产生了SER。此外，Ser至少由两种类型的质粒编码。特别是，一种编码Ser基因的质粒被称为携带Sed和Sej的pIB485相关质粒。
We identified and characterized a novel staphylococcal enterotoxin-like putative toxin, which is named SER. Nucleotide sequencing analysis of the ser gene revealed that ser was most closely related to the seg gene. The ser gene product, SER, was successfully expressed as a recombinant protein in an Escherichia coli expression system, and recombinant SER (rSER) showed significant T-cell stimulation activity. The SER production in ser-harboring Staphylococcus aureus strains was confirmed by Western blot analysis using anti-rSER antibody. Moreover, ser was seen to be encoded by at least two types of plasmids. In particular, one kind of plasmid encoding the ser gene has been known as a sed- and sej-carrying pIB485-related plasmid.