Luteolin, a flavonoid, inhibits AP-1 activation by basophils
Luteolin, a flavonoid, inhibits AP-1 activation by basophils
复制标题
DOI：
10.1016/j.bbrc.2005.11.157
复制
发表时间：
2006-02-03
影响因子：
3.1
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Hirano, T;Higa, S;Tanaka, T
包括毛地黄黄酮、芹菜素和非瑟酮的黄酮类化合物是嗜碱性粒细胞的IL-4合成和CD 40配体表达的抑制剂。本研究旨在寻找对IL-4表达具有更大抑制活性的化合物，并阐明类黄酮抑制其表达的分子机制。在检测的37种类黄酮和相关化合物中，阿亚宁、毛地黄黄酮和芹菜素是纯化的嗜碱性粒细胞响应抗IgE抗体和IL-3产生IL-4的最强抑制剂。木犀草素不抑制Syk或林恩在嗜碱性粒细胞中的磷酸化，也不抑制p54/46 SAPK/JNK、p38 NIAPK和p44/42 NIAPK在用A23187和PMA刺激的嗜碱性细胞系KU 812细胞中的活化。然而，毛地黄黄酮抑制磷酸化的c-Jun和AP-1的DNA结合活性的核裂解物从刺激的KU 812细胞。这些结果提供了类黄酮抑制IL-4的基本结构，并证明了类黄酮抑制AP-1活化的新作用。(c)2005年爱思唯尔公司All rights reserved.
Flavonoids including luteolin, apigenin, and fisetin are inhibitors of IL-4 synthesis and CD40 ligand expression by basophils. This study was done to search for compounds with greater inhibitory activity of IL-4 expression and to clarify the molecular mechanisms through which flavonoids inhibit their expression, Of the 37 flavonoids and related compounds examined, ayanin, luteolin, and apigenin were the strongest inhibitors of IL-4 production by purified basophils in response to anti-IgE antibody plus IL-3. Luteolin did not suppress Syk or Lyn phosphorylation in basophils, nor did suppress p54/46 SAPK/JNK, p38 NIAPK, and p44/42 NIAPK activation by a basophilic cell line, KU812 cells, stimulated with A23187 and PMA. However, luteolin did inhibit phosphorylation of c-Jun and DNA binding activity of AP-1 in nuclear lysates from stimulated KU812 cells. These results provide a fundamental structure of flavonoids for IL-4 inhibition and demonstrate a novel action of flavonoids that suppresses the activation of AP-1. (c) 2005 Elsevier Inc. All rights reserved.