Identification of Risk Factors Related to Poor Angiogenic Potency of Bone Marrow Cells From Different Patients
Identification of Risk Factors Related to Poor Angiogenic Potency of Bone Marrow Cells From Different Patients
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DOI：
10.1161/circulationaha.108.837039
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发表时间：
2009-09-15
期刊：
影响因子：
37.8
通讯作者：
Hamano, Kimikazu
中科院分区：
文献类型：
--
作者：
Li, Tao-Sheng;Kubo, Masayuki;Hamano, Kimikazu
背景-通过植入自体骨髓来源的细胞诱导的治疗性血管生成已用于治疗缺血性疾病。然而，由于细胞植入的结果因患者而异，因此有必要确定从这种治疗中获益最多的患者。方法和结果-我们收集了 25 名计划接受胸骨切开术以进行各种外科手术的患者的临床和实验室数据。然后，我们在手术过程中从胸骨中吸出骨髓细胞，并通过培养研究细胞的体外质量，并使用小鼠缺血肢体模型研究它们的体内血管生成能力。患者之间骨髓细胞的血管生成能力不同。衰老、肾衰竭、贫血以及甘油三酯、C反应蛋白、白细胞介素6和I型胶原交联N端肽(NTX)血清水平升高与骨髓细胞血管生成能力差显着相关。我们对这些风险因素进行评分，发现患者的风险评分与其骨髓细胞的血管生成能力之间存在很强的相关性（r=-0.883，P
Background-Therapeutic angiogenesis induced by the implantation of autologous bone marrow-derived cells has been used for the treatment of ischemic diseases. However, as the outcomes of cell implantation obviously vary among patients, it is essential to identify patients that would benefit the most from this treatment.Methods and Results-We collected clinical and laboratory data from 25 patients scheduled to undergo sternotomy for various surgical procedures. Then, we aspirated bone marrow cells from the sternum during the operation and investigated the cell quality in vitro by cultivation, and their angiogenic potency in vivo using an ischemic limb model of mice. The angiogenic potency of bone marrow cells differed among patients. Aging, renal failure, anemia, and high serum levels of triglyceride, C-reactive protein, interleukin-6, and type I collagen cross-linked N-telopeptide (NTX) significantly correlated with poor angiogenic potency of bone marrow cells. We assigned scores to these risk factors, and found a strong correlation between the risk scores of patients and the angiogenic potency of their bone marrow cells (r=-0.883, P