Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency.
Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency.
复制标题
角膜缘干细胞缺乏时角膜缘基底细胞密度降低。
DOI：
10.1016/j.ajo.2015.06.026
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发表时间：
2015-10
影响因子：
4.2
通讯作者：
Deng SX
中科院分区：
文献类型：
--
作者：
Chan EH;Chen L;Rao JY;Yu F;Deng SX
采用活体共聚焦激光扫描显微镜观察角膜缘干细胞缺乏症（LSCD）患者角膜缘基底上皮细胞密度的变化。10只正常眼作为对照。使用Heidelberg Retina Tomograph III罗斯托克角膜模块采集中央角膜、上级、鼻侧、下侧和颞侧利姆布斯的共焦成像。由两名独立观察员测量所有位置的基底细胞密度。正常组角膜基底细胞密度为9264 ±598个/mm 2，利姆布斯基底细胞密度为7120 ±362个/mm 2。与对照组相比，LSCD组角膜中的平均基底细胞密度降低31.0%（6389 ±1820个细胞/mm 2，p<0.001），利姆布斯中的平均基底细胞密度降低23.6%（5440 ±1123个细胞/mm 2，p<0.001）。LSCD晚期基底细胞密度有下降趋势。在扇形LSCD中，未受影响区域的基底细胞密度下降的程度与受影响区域相似（p>0.05）。与对照组相比，角膜基底细胞直径（14.7 μm）增加了24.6%，利姆布斯基底细胞直径（15.5 μm）增加了15.7%。在LSCD中，中央角膜和利姆布斯的基底细胞密度降低。LSC在全球范围内受到影响，基底细胞密度可用作在疾病过程的早期阶段测量LSC功能的参数。
To investigate changes in limbal basal epithelial cell density in eyes with limbal stem cell deficiency (LSCD) using in vivo confocal laser scanning microscopy retrospective observational comparative study A total of 43 eyes of 30 patients diagnosed with LSCD were included in the study. Ten eyes from normal subjects were included as control. Confocal imaging of the central cornea, and the superior, nasal, inferior and temporal limbus were collected using the Heidelberg Retina Tomograph III Rostock Corneal Module. Basal cell density in all locations was measured by two independent observers. The mean basal cell density of the normal group was 9264 ±598 cells/mm2 in the cornea and 7120 ±362 cells/mm2 in the limbus. In the LSCD group, the mean basal cell density in the cornea decreased 31.0% (6389 ±1820 cells/mm2, p<0.001) and in the limbus decreased 23.6% (5440 ±1123 cells/mm2, p<0.001) compared to that in the control. There was a trend of basal cell density decline in more advanced stage of LSCD. The basal cell density declined in the unaffected regions at a similar degree as that in the affected region in sectoral LSCD (p>0.05). The basal cell diameter increased by 24.6% in the cornea (14.7 μm) and by 15.7% in the limbus (15.5 μm) compared to the control. Basal cell density in both central cornea and limbus decreases in LSCD. LSCs are affected globally and basal cell density could be used as a parameter to measure LSC function at the early stages of the disease process.