The influence of platelets, plasma and red blood cells on functional haemostatic assays
The influence of platelets, plasma and red blood cells on functional haemostatic assays
复制标题
DOI：
10.1097/mbc.0b013e3283424911
复制
发表时间：
2011-04-01
影响因子：
1.1
通讯作者：
Ostrowski, Sisse R.
中科院分区：
文献类型：
--
作者：
Bochsen, Louise;Johansson, Par I.;Ostrowski, Sisse R.
功能性全血止血试验越来越多地用于指导输血治疗和监测医学治疗，也用于体外评估储存血小板的止血潜力。我们研究了分离和组合的细胞和血浆元素如何影响两种方法不同的分析，血栓造影（TEG）和阻抗聚集（Multiplate）。用血小板浓缩物、新鲜冷冻血浆和储存的红细胞体外制备富血小板血浆（200 × 10(9)/l）或纯血浆（0个血小板），分别添加或不添加红细胞（红细胞），红细胞比容为0、0.15或0.29。通过血小板储存液Intersol滤除血小板浓缩物中的血浆成分，研究了纯血小板。用Intersol对血浆进行过滤，读取血浆。TEG和Multiplate评价止血功能。在TEG中，红细胞数量的增加降低了凝块强度和凝块动力学（α角），在没有血小板的血浆/红细胞中最为明显。相比之下，血小板浓缩基质中的红细胞对弱激动剂（ADP和花生四烯酸）的反应增强了多板聚集。此外，从血小板浓缩物中去除血浆消除了TEG反应，减少了多板聚集反应，但将血浆读取到纯血小板浓缩物中恢复了反应。全血、血浆、血小板和红细胞中的每一种元素对Multiplate和TEG结果的影响不同。结果强调，在解释TEG和多板检测结果时，应考虑全血中所有细胞和血浆成分的浓度。血液凝固纤维蛋白溶解：167-175 (c) 2011 Wolters Kluwer Health垂直bar Lippincott Williams & Wilkins。
Functional whole blood haemostatic assays are used increasingly to guide transfusion therapy and monitor medical treatment and are also applied for in-vitro evaluations of the haemostatic potential of stored platelets. We investigated how the cellular and plasmatic elements, both isolated and combined, influenced the two methodologically different assays, thrombelastography (TEG) and impedance aggregometry (Multiplate). Platelet-rich plasma (200 X 10(9)/l) or pure plasma (0 platelets), with and without added red blood cells (RBCs), hematocrit 0, 0.15 or 0.29, were produced in vitro from platelet concentrates, fresh frozen plasma and stored RBC. Pure platelets were investigated by removing plasma components from platelet concentrates by diafiltration against the platelet storage solution Intersol. Plasma was readded by diafiltration against plasma in Intersol. Haemostatic function was evaluated by TEG and Multiplate. In the TEG, increasing amounts of RBC reduced clot strength and clot kinetics (alpha-angle), most markedly in plasma/RBC without platelets. In contrast, RBC in a platelet concentrate matrix enhanced Multiplate aggregation in response to weak agonists (ADP and arachidonic acid). Furthermore, removing plasma from platelet concentrates eliminated the TEG response and diminished the Multiplate aggregation response, but readding plasma to the pure platelet concentrates restored the response. Each of the elements in whole blood, plasma, platelets and RBC, affected the Multiplate and TEG results differently. The results emphasize that the concentrations of all cellular and plasmatic components in whole blood should be taken into account when interpreting results obtained by TEG and multiplate. Blood Coagul Fibrinolysis 22:167-175 (c) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.