Molecular breath-gas analysis by online mass spectrometry in mechanically ventilated patients: a new software-based method of CO2-controlled alveolar gas monitoring
Molecular breath-gas analysis by online mass spectrometry in mechanically ventilated patients: a new software-based method of CO2-controlled alveolar gas monitoring
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DOI：
10.1088/1752-7155/2/3/037010
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发表时间：
2008-09-01
影响因子：
3.8
通讯作者：
Schelling, G.
中科院分区：
文献类型：
--
作者：
Dolch, M. E.;Frey, L.;Schelling, G.
呼出气中挥发性有机化合物（VOC）的分析在研究和临床医学中提供了诊断潜力。呼气的质谱法允许VOC测量的浓度范围从万亿分之一到百万分之一。为了减少由于死腔混合物引起的与稀释相关的测量误差，精确识别呼气的呼气末阶段至关重要。我们使用了两个集成的MS系统的组合，包括一个传统的MS能够快速CO2跟踪控制的第二个，高灵敏度的MS的离子分子反应MS（IMR-MS）的基础上测量挥发性有机化合物。本研究旨在使用乙醛、丙酮、乙醇和异戊二烯作为目标VOC（IMR-MS化合物积分时间500 ms，循环时间2 ms，测量时间120 min），在12名通气患者中测试基于软件的CO2控制肺泡呼吸气体采样方法的适用性。CO2控制与混合吸气/呼气结果如下：乙醛71*（61-133）与63（47-87）;丙酮544*（208-1174）对比504（152-950）;乙醇133（99-166）对比123（108-185）;异戊二烯118*（69-253）对比58（44-112）（ppbv值作为中位数，25-75%; *p < 0.05，相对于混合吸气/呼气值）。所应用的基于软件的二氧化碳控制的呼气采样方法导致乙醛，丙酮和异戊二烯的浓度显着较高。
Analysis of volatile organic compounds (VOCs) in exhaled breath offers diagnostic potential in research and clinical medicine. Mass spectrometry of expiratory air allows VOC measurements in a concentration range from parts per trillion to parts per million. For the reduction of dilution-related measurement errors due to dead space admixture, the precise identification of the end-expiratory phase of expiration is essential. We used a combination of two integrated MS systems consisting of a conventional MS capable of fast CO2 tracing controlling a second, highly sensitive MS for the measurement of VOCs based on ion-molecule-reaction-MS (IMR-MS). This study intended to test the applicability of a software-based method of CO2-controlled alveolar breath-gas sampling in 12 ventilated patients using acetaldehyde, acetone, ethanol and isoprene as target VOCs (IMR-MS compound integration time 500 ms, cycle time 2 ms, measurement time 120 min). CO2-controlled versus mixed inspiratory/expiratory results are as follows: acetaldehyde 71* (61-133) versus 63 (47-87); acetone 544* (208-1174) versus 504 (152-950); ethanol 133 (99-166) versus 123 (108-185); isoprene 118* (69-253) versus 58 (44-112) (values in ppbv as medians with 25-75%; *p < 0.05 versus mixed inspiratory/expiratory values). The applied software-based CO2-controlled sampling method of expiratory air resulted in significant higher concentrations of acetaldehyde, acetone and isoprene.