Bipedal Locomotion in Crawling Cells
Bipedal Locomotion in Crawling Cells
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DOI：
10.1016/j.bpj.2009.10.058
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发表时间：
2010-03-17
影响因子：
3.4
通讯作者：
Theriot, Julie A.
中科院分区：
文献类型：
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作者：
Barnhart, Erin L.;Allen, Greg M.;Theriot, Julie A.
从有丝分裂到细胞运动的许多复杂的细胞过程都取决于细胞骨架产生力的能力。作用于弹性细胞骨架元件的力产生系统容易出现振荡不稳定。在这项工作中，我们测量了活动鱼上皮角膜细胞的自发形状和运动振荡。在持续极化的扇形电池中，电池体一侧后缘的收缩与另一侧的收缩异相，导致电池体周期性横向振荡。我们提出了角膜细胞振荡的物理描述，其中后缘的周期性回缩是与前缘弹性耦合的结果。与该模型的预测一致，观察到的振荡频率与细胞速度相关。此外，降低与基底的粘附强度会降低回缩所需的弹力，导致细胞以相对较低的速度以较高的频率振荡。这些结果表明，细胞前部运动和后部运动之间的简单弹性耦合可以产生细胞行为的大规模机械集成。
Many complex cellular processes from mitosis to cell motility depend on the ability of the cytoskeleton to generate force. Force-generating systems that act on elastic cytoskeletal elements are prone to oscillating instabilities. In this work, we have measured spontaneous shape and movement oscillations in motile fish epithelial keratocytes. In persistently polarized, fan-shaped cells, retraction of the trailing edge on one side of the cell body is out of phase with retraction on the other side, resulting in periodic lateral oscillation of the cell body. We present a physical description of keratocyte oscillation in which periodic retraction of the trailing edge is the result of elastic coupling with the leading edge. Consistent with the predictions of this model, the observed frequency of oscillation correlates with cell speed. In addition, decreasing the strength of adhesion to the substrate reduces the elastic force required for retraction, causing cells to oscillate with higher frequency at relatively lower speeds. These results demonstrate that simple elastic coupling between movement at the front of the cell and movement at the rear can generate large-scale mechanical integration of cell behavior.