Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology.
Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology.
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Laminin-521 通过光诱导细胞片技术促进大鼠骨髓间充质干细胞片的形成
DOI：
10.1155/2017/9474573
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发表时间：
2017
影响因子：
--
通讯作者：
Yang G
中科院分区：
文献类型：
--
作者：
Jiang Z;Xi Y;Lai K;Wang Y;Wang H;Yang G
大鼠骨髓间充质干细胞片层（rBMSC片层）是一种具有吸引力的细胞为基础的组织工程。然而，培养rBMSC片层的方法受到严重限制。为了获得完整的rBMSC片层，在本研究中使用光诱导细胞片层法。用（TL）或不用（TN）层粘连蛋白-521涂覆TiO 2纳米点膜。我们研究了层粘连蛋白-521在细胞片培养过程中对rBMSCs的影响。随后评估制造的rBMSC片以研究细胞片活力、再附着能力、细胞片厚度、I型胶原沉积和多谱系潜力。结果表明，层粘连蛋白-521能促进rBMSC在超融合条件下形成具有良好活性的片层。细胞片厚度从初始的26.7 ± 1.5 μm（第5天）增加至47.7 ± 3.0 μm（第10天）。此外，rBMSC片保持其成骨、成脂和成软骨分化的潜力。本研究为利用光诱导细胞片层技术获得rBMSC片层提供了一种新的策略。
Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.