Airway inflammatory responses to oxidative stress induced by prolonged low-dose diesel exhaust particle exposure from birth differ between mouse BALB/C and C57BL/6 strains
Airway inflammatory responses to oxidative stress induced by prolonged low-dose diesel exhaust particle exposure from birth differ between mouse BALB/C and C57BL/6 strains
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DOI：
10.1080/01902140701884406
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发表时间：
2008-03-01
影响因子：
1.7
通讯作者：
Kohyama, Tadashi
中科院分区：
文献类型：
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作者：
Li, Ying-Ji;Kawada, Tomoyuki;Kohyama, Tadashi
作者使用BALB/c和C57 BL/6小鼠品系，从出生开始，在气道炎症反应方面，寻找对长时间（6个月）低剂量（100 μ g/m3）柴油机排气颗粒（DEP）暴露的反应的遗传差异。组织学评估显示，炎症细胞仅在C57 BL/6小鼠中浸润血管周围区域。BALB/c小鼠支气管肺泡灌洗液（BAL）中载DEP的肺泡巨噬细胞计数显著高于C57 BL/6小鼠（P <0.05）。C57 BL/6小鼠的淋巴细胞和嗜酸性粒细胞计数显著高于BALB/c小鼠（P <0.05）。BALB/c小鼠血清免疫球蛋白（IG）IgG（1）和IgG（2）水平及支气管肺泡灌洗液（BAL）中单核细胞趋化蛋白（MCP）-1水平均显著高于C57 BL/6小鼠。C57 BL/6小鼠BALF中IL-12水平显著高于BALB/c小鼠，而IL-13水平显著低于C57 BL/6小鼠。C57 BL/6小鼠肺组织中谷胱甘肽S-转移酶（GST）mRNA表达和蛋白质生成量显著低于BALB/c小鼠，而8-羟基脱氧鸟苷（8-OHdG）水平显著高于BALB/c小鼠。总之，长时间低剂量DEP暴露诱导的气道炎症反应，小鼠品系之间显着不同，这些差异是由宿主防御反应的差异DEP暴露诱导的氧化应激，并可能是有用的生物标志物的发展。
The authors used BALB/c and C57BL/6 mouse strains to search for genetically based differences in response to prolonged (6 months) low-dose (100 mu g/m(3)) diesel exhaust particle (DEP) exposure from birth in terms of airway inflammatory responses. Histopathological assessment showed that inflammatory cells infiltrated the perivascular areas only in C57BL/6 mice. The count of DEP-laden alveolar macrophages in bronchoalveolar lavage (BAL) fluid was significantly greater in BALB/c mice (P < .05) than in C57BL/6 mice. The lymphocyte and eosinophil count in BAL fluid was significantly greater in C57BL/6 mice (P < .05) than in BALB/c mice. Immunoglobulin (Ig) IgG(1) and IgG(2) levels in serum, and the monocyte chemoattractant protein (MCP)-1 level in BAL fluid were significantly greater in BALB/c mice than in C57BL/6 mice. The interleukin (IL)-12 level in BAL fluid was significantly greater in C57BL/6 mice than in BALB/c mice, but the IL-13 level in BAL fluid was significantly less in BALB/c mice than in C57BL/6 mice. Glutathione S-transferase (GST) mRNA expression and protein production in lung tissues were significantly lower in C57BL/6 mice than in BALB/c mice, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) level in the lung tissues were significantly greater in C57BL/6 mice than in BALB/c mice. In conclusion, prolonged low-dose DEP exposure induces airway inflammatory responses that differ remarkably among mouse strains; these differences are caused by differences in the host defense response to the oxidative stress induced by DEP exposure and may be useful in the development of biomarkers.