Expression pattern of MRP in human tissues and adult solid tumor cell lines.
Expression pattern of MRP in human tissues and adult solid tumor cell lines.
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MRP 在人体组织和成人实体瘤细胞系中的表达模式。
DOI：
10.1093/jnci/87.16.1256
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Chan,A
中科院分区：
文献类型：
--
作者：
Kruh,GD;Gaughan,KT;Godwin,A;Chan,A
细胞对细胞毒性药物的抗性是癌症治疗的主要障碍。耐药癌细胞可以显示多药耐药表型，涉及同时耐一系列天然产物细胞毒性药物。一种形式的多药耐药是由P-糖蛋白的表达引起的，P-糖蛋白是MDR 1（也称为PGY 1）基因的170 kd蛋白产物（7-5）。P-糖蛋白是一种三磷酸腺苷结合盒（ABC）转运蛋白，作为外排泵降低细胞内药物浓度。尽管P-糖蛋白表达似乎与某些血液恶性肿瘤的不良预后相关（4-7），但与大多数成人实体瘤的化疗敏感性或临床结局的相关性尚未确定。最近，从耐药细胞系中分离出另一种ABC转运蛋白多药耐药相关蛋白（MRP）（8）。我们（9）和其他研究者（10）已经报道了转染研究，这些研究已经确定MRP的表达赋予多药耐药性。虽然已经有关于白血病、神经母细胞瘤和甲状腺癌中MRP表达的报道（11-15），但尚未描述MRP在成人中常见的实体癌中的表达模式。因此，我们在建立的肿瘤细胞系中检测了MRP的表达。
Cellular resistance to cytotoxic drugs is a major obstacle to cancer treatment. Resistant cancer cells can display a multidrug-resistant phenotype that involves simultaneous resistance to a spectrum of natural-product cytotoxic drugs. One form of multidrug resistance results from the expression of P-glycoprotein, the 170-kd protein product of the MDR1 (also known as PGY1) gene (7-5). P-glycoprotein, an adenosine triphosphate-binding cassette (ABC) transporter, functions as an efflux pump to decrease intracellular drug concentrations. Although P-glycoprotein expression appears to be associated with poor prognosis in some hematologic malignancies (4-7), associations with either chemosensitivity or clinical outcome have yet to be established for most solid tumors in adults. Recently, multidrug resistance-associated protein (MRP), another ABC transporter, was isolated from a drug resistant cell line (8). We (9) and other investigators (10) have reported transfection studies that have established that expression of MRP confers multidrug resistance. While there have been reports (11-15) on MRP expression in leukemias, neuroblastoma, and thyroid cancer, the expression pattern of MRP in commonly occurring solid cancers in adults has not been described. We therefore examined MRP expression in a spectrum of tumor cell lines established