Interaction of a macrocyclic bisacridine with DNA.
Interaction of a macrocyclic bisacridine with DNA.
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大环双吖啶与 DNA 的相互作用。
DOI：
10.1021/bi00501a009
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Wilson,WD
中科院分区：
文献类型：
--
作者：
Veal,JM;Li,Y;Zimmerman,SC;Lamberson,CR;Cory,M;Zon,G;Wilson,WD
材料和方法可见光谱学。用Cary 2200分光光度计进行可见光谱分析。将含有0.1M氯化钠的MES缓冲液(10 mM MES，1 mM EDTA，pH 6.5)加入10 cm的试管中，记录基线光谱。然后用汉密尔顿注射器将浓缩的SDM(413 nm=18000 M“L厘米”1)加入到试管中，在500-320 nm范围内记录吸收光谱。然后将小体积的浓缩聚合物DNA溶液滴定到SDM溶液中，然后重新扫描吸收光谱
Materials and Methods Visible Spectroscopy. Visible spectroscopy was performed by using a Cary 2200 spectrophotometer. MES buffer (10 mM MES, 1 mM EDTA, pH 6.5) containing 0.1 M NaCl was added to a 10-cm cuvette, and base-line spectra were recorded. Concentrated SDM (< 4l3nm= 18000 M “l cm" 1) was then added to the cuvette with a Hamilton syringe to a concentration of 5.5 µ, and absorption spectra were recorded from 500 to 320 nm. Small volumes of a solution of concentrated polymer DNA in MES buffer/0.1 M NaCl were then titrated into the SDM solution and the absorption spectrum rescanned after