Effect of Phosphoric Acid on the Degradation of Human Dentin Matrix
Effect of Phosphoric Acid on the Degradation of Human Dentin Matrix
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DOI：
10.1177/0022034512466264
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发表时间：
2013-01-01
影响因子：
7.6
通讯作者：
Pashley, D. H.
中科院分区：
文献类型：
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作者：
Tezvergil-Mutluay, A.;Mutluay, M.;Pashley, D. H.
本研究确定牙本质蛋白水解酶是否会被用于酸洗牙本质粘合剂的磷酸(PA)变性。牙本质梁用EDTA完全脱矿30d。我们通过将脱矿牙本质梁暴露在1、10或37质量%的PA中15秒或15分钟来对实验组进行酸蚀处理。对照梁不暴露于PA，而是在模拟体液中孵育3天，以测定其总的内源性端肽酶活性，通过它们从不溶性牙本质胶原中溶解C端交联端肽ICTP和CTX的能力。对照组释放6.1+/-0.8ngICTP和0.6+/-0.1ngCTX/mg干重/3d。阳性对照受试者预先孵育在对氨基苯基汞醋酸酯中，一种已知能激活proMMPs的化合物，释放大致相同数量的ICTP多肽，但释放的CTX明显减少。在1、10或37质量%PA中浸泡15秒或15分钟的电子束释放ICTP和CTX的量与对照组释放的量相似(p>；0.05)。在基质金属蛋白酶抑制剂Galardin或组织蛋白酶抑制剂E-中孵育的BEAM分别阻断了ICTP和CTX的大部分释放。结论：PA不影响牙本质基质的内源性基质金属蛋白酶和组织蛋白酶活性。
This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We "acid-etched" experimental groups by exposing the demineralized dentin beams to 1, 10, or 37 mass% PA for 15 sec or 15 min. Control beams were not exposed to PA but were incubated in simulated body fluid for 3 days to assay their total endogenous telopeptidase activity, by their ability to solubilize C-terminal crosslinked telopeptides ICTP and CTX from insoluble dentin collagen. Control beams released 6.1 +/- 0.8 ng ICTP and 0.6 +/- 0.1 ng CTX/mg dry-wt/3 days. Positive control beams pre-incubated in p-aminophenylmercuric acetate, a compound known to activate proMMPs, released about the same amount of ICTP peptides, but released significantly less CTX. Beams immersed in 1, 10, or 37 mass% PA for 15 sec or 15 min released amounts of ICTP and CTX similar to that released by the controls (p > 0.05). Beams incubated in galardin, an MMP inhibitor, or E-64, a cathepsin inhibitor, blocked most of the release of ICTP and CTX, respectively. It is concluded that PA does not denature endogenous MMP and cathepsin activities of dentin matrices.