First crAss-Like Phage Genome Encoding the Diversity-Generating Retroelement (DGR)
First crAss-Like Phage Genome Encoding the Diversity-Generating Retroelement (DGR)
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DOI：
10.3390/v12050573
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发表时间：
2020-05-01
期刊：
影响因子：
4.7
通讯作者：
Tikunov, Artem
中科院分区：
文献类型：
--
作者：
Morozova, Vera;Fofanov, Mikhail;Tikunov, Artem
在一名健康志愿者的粪便病毒体内发现了一种新的编码多样性生成逆转录因子（DGR）的类草基因组。该噬菌体的基因组被称为噬菌体LMMB，属于α噬菌体亚科的候选属I。crAssphage LMMB的DGR-cassette包含了所有必需元件：编码逆转录酶（RT）的基因，编码尾领纤维蛋白的靶基因（TG），以及分别在VR和TR的3 '端具有IMH （initiation of mutagenic homing）和IMH*序列的可变重复序列和模板重复序列（VR和TR）。DGR-cassette的结构为TG-VR(IMH)-TR(IMH*)-RT，盒中没有辅助变量行列式（avd）。对1属的91个基因组和基因组片段的分析表明，15个（16%）基因组的DGR结构与crAssphage LMMB相同，66个基因组包含不完整的DGR磁带或部分DGR元件。
A new crAss-like genome encoding diversity-generating retroelement (DGR) was found in the fecal virome of a healthy volunteer. The genome of the phage referred to as the crAssphage LMMB, belonged to the candidate genus I of the AlphacrAssvirinae subfamily. The DGR-cassette of the crAssphage LMMB contained all the essential elements: the gene encoding reverse transcriptase (RT), the target gene (TG) encoding the tail-collar fiber protein, and variable and template repeats (VR and TR) with IMH (initiation of mutagenic homing) and IMH* sequences at the 3 ' -end of the VR and TR, respectively. Architecture of the DGR-cassette was TG-VR(IMH)-TR(IMH*)-RT and an accessory variable determinant (avd) was absent from the cassette. Analysis of 91 genomes and genome fragments from genus I of the AlphacrAssvirinae showed that 15 (16%) of the genomes had DGRs with the same architecture as the crAssphage LMMB, while 66 of the genomes contained incomplete DGR-cassettes or some elements of the DGR.