Miniature synaptic events maintain dendritic spines via AMPA receptor activation
Miniature synaptic events maintain dendritic spines via AMPA receptor activation
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DOI：
10.1038/4548
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发表时间：
1999-01-01
影响因子：
25
通讯作者：
Thompson, SM
中科院分区：
文献类型：
--
作者：
McKinney, RA;Capogna, M;Thompson, SM
我们研究了突触释放的谷氨酸对突触后结构的影响，通过比较去传入，受体拮抗剂和谷氨酸释放的阻断剂在海马脑片培养中的作用。CA1锥体细胞棘密度和长度减少后横断的Schaffer侧支和AMPA受体拮抗剂或肉毒杆菌毒素应用于unilesed文化。同时AMPA应用可防止病变或肉毒杆菌毒素引起的脊柱丢失。河豚毒素不影响棘密度。因此，突触释放的谷氨酸通过作用于AMPA受体对棘发挥营养作用。我们的结论是自发囊泡谷氨酸释放AMPA受体激活是足以维持树突棘。
We investigated the influence of synaptically released glutamate on postsynaptic structure by comparing the effects of deafferentation, receptor antagonists and blockers of glutamate release in hippocampal Slice cultures. CA1 pyramidal cell spine density and length decreased after transection of Schaffer collaterals and after application of AMPA receptor antagonists or botulinum toxin to unlesioned cultures. Loss of spines induced by lesion or by botulinum toxin was prevented by simultaneous AMPA application. Tetrodotoxin did not affect spine density. Synaptically released glutamate thus exerts a trophic effect on spines by acting at AMPA receptors. We conclude that AMPA receptor activation by spontaneous vesicular glutamate release is sufficient to maintain dendritic spines.