The use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide-based colorimetric assay in the viability analysis of the filamentous cyanobacterium Arthrospira platensis
The use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide-based colorimetric assay in the viability analysis of the filamentous cyanobacterium Arthrospira platensis
复制标题
基于 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物的比色测定在丝状蓝藻钝顶节旋藻活力分析中的应用
DOI：
10.1093/bbb/zbaa050
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Shiraishi;H. and Toyoda;A.
研究了3-(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑溴化法对一种有工业价值的丝状钝顶螺旋藻的适用性。当它被应用于ToA时。PlatenssNIES-39，仅定量检测到10个活毛状体。然而，根据实验条件的不同，它也会产生人工生存信号。这一结果应该有助于澄清四甲基偶氮唑蓝试验在生存分析中的范围和限度。
The applicability of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to an industrially valuable filamentous cyanobacteriumArthrospira platensiswas examined. When it was applied toA. platensisNIES-39, as few as 10 viable trichomes were quantitatively detected. However, depending on the experimental conditions, it also generated artifactual viability signals. The results should help clarify the scope and limits of the MTT assay in viability analysis.