Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity.
Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity.
复制标题
分离大鼠端脑神经外植体以测定 Shh GABA 能中间神经元分化诱导活性。
DOI：
10.1007/978-1-59745-516-9_4
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Kohtz,JhumkuD
中科院分区：
文献类型：
--
作者：
Mady,Rina;Kohtz,JhumkuD
Multiple assays for Shh activity using cell lines, primary cultures, and explanted tissue have been described. We first described the use of E11.5 rat dorsal telencephalic explants to assay Shh ventralizing and differentiation-inducing activity in Kohtz et al. (1). Using this assay, we subsequently showed that N-lipid modification is critical for Shh activity in the telencephalon (2). In vivo assays for lipid-modified Shh support the results of our E11.5 telencephalic neural explant assay (2). More recently, the method of isolating telencephalic explants was improved by an intraocular grid, increasing both its accuracy and reproducibility (3). Shh induces the expression of the following ventral telencephalic markers: MASH-1, the Dlx’s, and Islet 1/2. Therefore, this assay for Shh induction of GABAergic interneurons defines a competent, but naïve region within the E11.5 dorsal telencephalon, allowing the study of GABAergic interneuron induction and differentiation from an unspecified progenitor population.
