Development and evaluation of a multiple-locus variable-number tandem-repeats analysis assay for subtyping Salmonella Typhi strains from sub-Saharan Africa.
Development and evaluation of a multiple-locus variable-number tandem-repeats analysis assay for subtyping Salmonella Typhi strains from sub-Saharan Africa.
复制标题
开发和评估用于对撒哈拉以南非洲伤寒沙门氏菌菌株进行亚型分型的多位点可变数量串联重复分析测定法。
DOI：
10.1099/jmm.0.000526
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发表时间：
2017
影响因子：
3
通讯作者：
Tau NP
中科院分区：
文献类型：
--
作者：
Tau NP
高克隆性伤寒沙门氏菌亚种的分子流行病学研究在暴发检测和追踪疾病传播中是重要的。在这项研究中，我们开发和评估了多位点可变数目串联重复序列（VNTR）分析（MLVA）检测表征ofS.伤寒菌株从撒哈拉以南Africa.Methodology。通过检测50 S的集合，开发了MLVA测定法，用于毛细管电泳系统。伤寒杆菌。本伤寒沙门氏菌菌株组包括6个暴发相关分离株和44个流行病学无关分离株。结果：MLVA检测将50株伤寒沙门氏菌分为47个MLVA谱型，PFGE分析显示34个脉冲型。MLVA显示出更高的区分能力（Simpson多样性指数（DI）0.998 [95%置信区间（CI）0.995-1.000）]相比，脉冲场凝胶电泳[Simpson的DI 0.984（95% CI 0.974-0.994）]。结论：本研究中提出的MLVA检测是一种简单，快速和更容易获得的工具，作为一个很好的替代其他分子分型方法为S.型。
Purpose.Molecular epidemiological investigations of the highly clonalSalmonella entericasubspeciesentericaserovar Typhi (S.Typhi) are important in outbreak detection and in tracking disease transmission. In this study, we developed and evaluated a multiple-locus variable-number tandem-repeats (VNTR) analysis (MLVA) assay for characterization ofS.Typhi isolates from sub-Saharan Africa.Methodology.Twelve previously reported VNTR loci were evaluated and an MLVA assay consisting of five polymorphic loci was adopted. The MLVA assay was developed for use on capillary electrophoresis systems by testing a collection of 50S. Typhi isolates. ThisS.Typhi strain panel consisted of six outbreak related isolates and 44 epidemiologically unlinked isolates. Amongst these were nineS.Typhi haplotype H58 isolates.Results.The MLVA assay characterized the 50 isolates into 47 MLVA profiles while PFGE analysis of the same isolates revealed 34 pulsotypes. MLVA displayed higher discriminatory power (Simpson’s index of diversity (DI) 0.998 [95 % confidence interval (CI) 0.995–1.000)] as compared to pulsed-field gel electrophoresis [Simpson’s DI 0.984 (95 % CI 0.974–0.994)].Conclusion.The MLVA assay presented in this study is a simple, rapid and more accessible tool that serves as a good alternative to other molecular subtyping methods forS.Typhi.