Effects of atrazine and chlorpyrifos on DNA methylation in the liver, kidney and gill of the common carp (Cyprinus carpio L.).
Effects of atrazine and chlorpyrifos on DNA methylation in the liver, kidney and gill of the common carp (Cyprinus carpio L.).
复制标题
阿特拉津和毒死蜱对鲤鱼 (Cyprinus carpio L.) 肝脏、肾脏和鳃 DNA 甲基化的影响。
DOI：
10.1016/j.ecoenv.2014.06.011
复制
发表时间：
2014
影响因子：
6.8
通讯作者：
Shiwen Xu
中科院分区：
文献类型：
--
作者：
Chao Wang;Ziwei Zhang;Hai;Fuqing Zhao;Liangliang Wang;Xiaolong Wang;H. Xing;Shiwen Xu
农药暴露多次与癌症有关，尽管这种关联背后的分子机制在很大程度上尚未确定。DNA甲基化异常在某些疾病的发生、发展过程中起着重要作用。然而，农药对鲤鱼DNA甲基化的影响却知之甚少。本研究以鲤鱼（Cyprinus carpio L.）为材料，研究了鲤鱼肝脏、肾脏和鳃中DNA甲基化水平、DNA甲基转移酶（DNMTs）和甲基化CpG结合蛋白DNA结合域蛋白2（MBD 2）的mRNA水平。阿特拉津（ATR）和毒死蜱（CPF）单独或联合暴露40天后，和40天的恢复期。将鲤鱼幼鱼暴露于不同浓度的ATR（4.28、42.8和428 μg/L）、CPF（1.16、11.6和116 μg/L）和ATR/CPF混合物（1.13、11.3和113 μg/L）。结果表明，ATR和CPF单独或联合暴露40 d后，所有组织的基因组DNA甲基化水平下降。此外，DNMTs的mRNA表达在所有处理组中均下调。而MBD 2的mRNA表达上调。这些结果表明，长期暴露于ATR，CPF和ATR/CPF混合物可以破坏基因组DNA。这可能暗示DNA甲基化参与了ATR和CPF对鲤鱼的毒性作用。
Pesticide exposure has repeatedly been associated with cancers, although the molecular mechanisms behind this association are largely undetermined. Abnormal DNA methylation plays a key role in the process of some disease. However, little was known about the effect of pesticides on DNA methylation in the common carp. In this study, we investigated the mRNA levels of DNA methyltransferases (DNMTs) and methyl-CpG-binding protein DNA-binding domain protein 2 (MBD2) as well as the DNA methylation levels in the liver, kidney and gill of the common carp (Cyprinus carpio L.) after 40-d exposure to atrazine (ATR) and chlorpyrifos (CPF) alone or in combination, and a 40-d recovery period. Juvenile common carp were exposed to various concentrations of ATR (at concentrations of 4.28, 42.8 and 428 μg/L), CPF (1.16, 11.6 and 116 μg/L), and an ATR/CPF mixture (at concentrations of 1.13, 11.3 and 113 μg/L). The results revealed that the levels of genomic DNA methylation decreased in all tissues after 40 d of exposure to ATR and CPF either individually or in combination. Moreover, the mRNA expression of DNMTs was down-regulated in all treatment groups. In contrast, the mRNA expression of MBD2 was up-regulated. These results demonstrated that long-term exposure to ATR, CPF and ATR/CPF mixtures could disrupt genomic DNA. It might imply that DNA methylation is involved in the toxicity caused by ATR and CPF in the common carp.
苯并暴露后，对斑马鱼胚胎和幼虫中特定启动子DNA甲基化的影响。
DOI：
10.1016/j.cbpc.2014.02.005
发表时间：
2014-06
影响因子：
3.9
作者：
Corrales, J.;Fang, X.;Thornton, C.;Mei, W.;Barbazuk, W. B.;Duke, M.;Scheffler, B. E.;Willett, K. L.
通讯作者：
Willett, K. L.
影响因子：
4.3
作者：
Zhang X;Wallace AD;Du P;Lin S;Baccarelli AA;Jiang H;Jafari N;Zheng Y;Xie H;Soares MB;Kibbe WA;Hou L
通讯作者：
Hou L