Development and Characterization of a Strain of Babesia gibsoni Resistant to Diminazene Aceturate In Vitro
Development and Characterization of a Strain of Babesia gibsoni Resistant to Diminazene Aceturate In Vitro
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DOI：
10.1292/jvms.09-0535
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发表时间：
2010-06-01
影响因子：
1.2
通讯作者：
Takiguchi, Mitsuyoshi
中科院分区：
文献类型：
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作者：
Hwang, Shiang-Jyi;Yamasaki, Masahiro;Takiguchi, Mitsuyoshi
我们试图在体外培养一株对抗吉氏巴贝斯虫药物醋酸亚胺氮烯（DA）具有耐药性的吉氏巴贝斯虫。由于DA敏感的B. gibsoni菌株在含1 ng/ml DA的培养基中能存活和增殖，DA浓度从1逐渐增加到200 ng/ml。结果表明，在含200 ng/ml DA的培养基中，疟原虫能存活和增殖，远远高于DA对B的半数抑制浓度（IC 50）。吉布森尼。随后，将这些寄生虫从红细胞中取出并直接暴露于200 ng/ml DA，它们能够存活并侵入新鲜红细胞，通过DA敏感的B。Gibsoni菌株不能存活。基于这些结果，在200 ng/ml DA中培养的寄生虫被确定为DA抗性B。吉布森菌株。此外，克林霉素、多西环素和喷他脒对DA耐药B的IC 50水平。Gibsoni菌株进行了测定。克林霉素、强力霉素和喷他脒对DA耐药株的IC_（50）值均高于对DA敏感株的IC_（50）值。喷他脒对抗性菌株的IC_（50）远大于对DA敏感的菌株。这些结果表明，抗DA的B. Gibsoni菌株不仅对DA耐药，而且对其它抗巴贝斯虫药物也有耐药性。总之，我们成功地开发了一种抗DA的B。gibsoni菌株体外培养。
We attempted to develop a strain of Babesia gibsoni resistant to diminazene aceturate (DA), an anti-babesial drug, in vitro. Since the DA-sensitive B. gibsoni strain could survive and proliferate in culture medium containing 1 ng/ml DA, the concentration of DA was gradually increased from 1 to 200 ng/ml. The results showed that the parasites could survive and proliferate in the medium containing 200 ng/ml DA, which was much higher than the 50% inhibitory concentration (IC50) of DA for B. gibsoni. Subsequently, these parasites were removed from erythrocytes and exposed directly to 200 ng/ml DA. They were able to survive and invade fresh erythrocytes, though the DA-sensitive B. gibsoni strain did not survive. Based on these results, the parasites cultured within 200 ng/ml DA were determined to be a DA-resistant B. gibsoni strain. In addition, the IC50 levels of clindamycin, doxycycline and pentamidine for the DA-resistant B. gibsoni strain were determined. The IC50 levels of clindamycin, doxycycline and pentamidine for the DA-resistant strain were higher than those for the DA-sensitive strain. The IC50 of pentamidine for the resistant strain was much greater than that for the DA-sensitive strain. These results indicated that the DA-resistant B. gibsoni strain could have resistance not only to DA, but also to other anti-babesial drugs. In conclusion, we successfully developed a DA-resistant B. gibsoni strain in vitro.