Improved lung function by urokinase infusion in canine lung transplantation using non-heart-beating donors.
Improved lung function by urokinase infusion in canine lung transplantation using non-heart-beating donors.
复制标题
在使用无心跳供体的犬肺移植中通过尿激酶输注改善肺功能。
DOI：
10.1016/0003-4975(95)00188-q
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
N. Shimizu
中科院分区：
文献类型：
--
作者：
Y. Umemori;H. Date;K. Uno;M. Aoe;A. Ando;N. Shimizu
我们利用犬同种异体左肺移植模型评价了尿激酶对供体肺肺微血栓形成的影响。供体动物不经肝素化，静脉注射氯化钾处死动物，随机分为3组。第1组(n=6)，将身体置于室温下1h后，用低钾葡聚糖溶液冲洗肺块，取肺。供体肺在8℃下保存3h，第2组(n=6)供体肺处理同第1组，只是身体在室温下保存2小时，而不是在取肺前1小时。组3(n=6)，供肺处理同组2，大剂量尿激酶(12万IU)经低钾葡萄糖溶液冲洗后注入主肺动脉。各组大鼠左肺移植后均结扎右肺动脉，再灌注后随访6h。同时测定供体肺组织中纤维蛋白降解产物的含量。第1组受体动物均存活6小时，气体交换良好，血流动力学稳定。移植前供体肺纤维蛋白降解产物水平明显高于供体肺组织。本研究支持尿激活酶通过对供体肺内微血栓形成的纤溶作用改善移植后肺功能的理论。
We evaluated the effect of urokinase on pulmonary microthrombi formation of the donor lung using a canine cadaver left lung allotransplantation model. Donor animals were sacrificed with an intravenous injection of potassium chloride without heparinization and were divided into three groups. In group 1 (n = 6), cadavers were left at room temperature for 1 hour, and lung retrieval was then performed after flushing the lung block with low potassium—dextran-glucose solution. Donor lungs were stored for 3 hours at 8°C. In group 2 (n = 6), donor lungs were treated as in group 1 except that the cadavers were left at room temperature for 2 hours instead of 1 hour before lung retrieval. In group 3 (n = 6), donor lungs were treated as in group 2 except that high-dose urokinase (120,000 IU) was injected into the main pulmonary artery after flushing with low-potassium—dextrose-glucose solution. In all groups after left lung transplantation, the right pulmonary artery was ligated, and recipient animals were followed up for 6 hours after reperfusion. The fibrin degradation product level in the donor lung tissue was also measured. All recipient animals in group 1 survived the 6-hour observation period with excellent gas exchange and stable hemodynamics. Group 3 had significantly better gas exchange than group 2 and similar cardiopulmonary function as group 1. The fibrin degradation product level in the donor lungs before transplantation was significantly higher in group 3 than in group 2. This study supports the theory that urokinase improves lung function after cadaver lung transplantation by fibrinolytic action on pulmonary microthrombi formation in the cadaver donor lungs.
DOI：
10.1016/s0022-5223(19)34231-x
发表时间：
1993-03
期刊：
The Journal of thoracic and cardiovascular surgery
影响因子：
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作者：
Hiroshi Date;A. Matsumura;Jill K. Manchester;Hidefumi Obo;Oriane Lima;Joshua M. Cooper;Sudhir Sundaresan;Oliver H. Lowry;Joel D. Cooper
通讯作者：
Hiroshi Date;A. Matsumura;Jill K. Manchester;Hidefumi Obo;Oriane Lima;Joshua M. Cooper;Sudhir Sundaresan;Oliver H. Lowry;Joel D. Cooper