Role of the Guanine Nucleotide Exchange Factor Rom2 in Cell Wall Integrity Maintenance of Aspergillus fumigatus
Role of the Guanine Nucleotide Exchange Factor Rom2 in Cell Wall Integrity Maintenance of Aspergillus fumigatus
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DOI：
10.1128/ec.00246-12
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发表时间：
2013-02-01
期刊：
影响因子：
--
通讯作者：
Wagener, Johannes
中科院分区：
文献类型：
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作者：
Samantaray, Sweta;Neubauer, Michael;Wagener, Johannes
烟曲霉是一种霉菌，也是侵袭性曲霉病的病原，是一种致死性很高的全身性疾病。最近，我们鉴定并鉴定了与该病原菌的细胞壁完整性(CWI)信号有关的三个胁迫感受器，即Wsc1、Wsc3和MIDA。在这里，我们对Rom2进行了功能分析，Rom2是一种鸟嘌呤核苷酸交换因子，对烟曲霉细胞壁的完整性具有重要作用。有条件的rom2突变体在抑制条件下有严重的生长缺陷，并整合了三个细胞壁完整性感受器突变体的所有表型，例如Delta wsc1突变体的棘球蛋白敏感性和Delta Mida突变体的刚果红、钙氟白和热敏性。Rom2与Rho1相互作用，在菌丝顶端表现出类似的细胞内分布。我们的结果将Rom2放置在细胞表面应力传感器Wsc1、Wsc3、MIDA和Rho1及其下游效应丝裂原激活蛋白(MAP)激酶模块Bock1-Mkk2-MpkA之间。
Aspergillus fumigatus is a mold and the causal agent of invasive aspergillosis, a systemic disease with high lethality. Recently, we identified and functionally characterized three stress sensors implicated in the cell wall integrity (CWI) signaling of this pathogen, namely, Wsc1, Wsc3, and MidA. Here, we functionally characterize Rom2, a guanine nucleotide exchange factor with essential function for the cell wall integrity of A. fumigatus. A conditional rom2 mutant has severe growth defects under repressive conditions and incorporates all phenotypes of the three cell wall integrity sensor mutants, e. g., the echinocandin sensitivity of the Delta wsc1 mutant and the Congo red, calcofluor white, and heat sensitivity of the Delta midA mutant. Rom2 interacts with Rho1 and shows a similar intracellular distribution focused at the hyphal tips. Our results place Rom2 between the cell surface stress sensors Wsc1, Wsc3, MidA, and Rho1 and their downstream effector mitogen-activated protein (MAP) kinase module Bck1-Mkk2-MpkA.