Genome Wide Identi�cation and Characterization of Light-Harvesting Chloro a/b Binding Genes Reveals their Potential Role in Enhancing Drought Tolerance in Gossypium hirsutum
Genome Wide Identi�cation and Characterization of Light-Harvesting Chloro a/b Binding Genes Reveals their Potential Role in Enhancing Drought Tolerance in Gossypium hirsutum
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发表时间：
2020
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通讯作者：
Teame Gereziher Mehari;Yanchao Xu;R. Magwanga;Muhammad Umer;J. N. Kirungu;Xiaoyan Cai;Yuqing Hou;Yuhong Wang;Shuxun Yu;Kunbo Wang;Zhongli Zhou;Fang Liu
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作者：
Teame Gereziher Mehari;Yanchao Xu;R. Magwanga;Muhammad Umer;J. N. Kirungu;Xiaoyan Cai;Yuqing Hou;Yuhong Wang;Shuxun Yu;Kunbo Wang;Zhongli Zhou;Fang Liu
背景棉花是一种重要的经济作物，是一种宝贵的天然资源。由于非生物胁迫的影响，其生产经历了急剧的失败，其中最重要的是干旱。此外，植物已经进化出自我防御机制，以对抗干旱，盐，寒冷等非生物因素的影响。胁迫应答转录因子如三螺旋（trihelix）、结节起始样蛋白（nodule-inception-like protein，NLP）、晚期胚胎发生丰富（late embryogenesis abundant，莱亚）蛋白等的进化在提高对几种形式的非生物胁迫特征的抗性方面显示出积极的响应。结果在全基因组范围内鉴定了干旱胁迫下棉花中捕光叶绿素a/B结合（LHC）基因的作用。在棉花基因组中发现了109个LHC基因编码的蛋白质，其中陆地棉、棉和棉中分别发现了55、27和27个LHC基因。arboreum，和G. raimondii）。这些基因编码的蛋白质不均匀地分布在不同的染色体上。Ka/Ks值小于1，表明基因家族的负选择。基因的差异表达排列显示，相对于叶和茎组织，大多数基因在根组织中高度上调。此外，M85是一个相对耐旱的种质，它诱导了更多的基因。结论：这些结果为LHC基因在提高棉花耐旱性中的可能作用提供了证据，并可供棉花育种者探索，以释放更耐旱的棉花种质。
Background Cotton is an important commercial crop for its valuable source of natural �ber. Its production has undergone a sharp failure because of abiotic stress in�uences, of signi�cance is drought. Moreover, plants have evolved self-defense mechanisms against the effects of several ways of abiotic factors like drought, salt, cold among others. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), the late embryogenesis abundant (LEA) proteins among others have shown positive response in improving resistance to several forms of abiotic stress features. Results Genome wide identi�cation and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes was carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed in various chromosomes. The Ka/Ks values were less than one, and an indication of negative selection of the gene family. differential expression arrangement of genes was showed with the majority of the genes being highly upregulated in the root tissues in relative to leave and stem tissues. Moreover, more genes were induced in M85 a relative drought tolerant germplasm. Conclusion: The results provide proof of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton germplasms.