Prenatal exposure to diesel exhaust impairs mouse spermatogenesis
Prenatal exposure to diesel exhaust impairs mouse spermatogenesis
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DOI：
10.1080/08958370601069257
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发表时间：
2007-03-01
影响因子：
2.1
通讯作者：
Takeda, Ken
中科院分区：
文献类型：
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作者：
Ono, Naoka;Oshio, Shigeru;Takeda, Ken
探讨产前接触柴油废气(DE)对孕妇的影响。20只妊娠ICR小鼠从产后第2天至第16天，以1.0 mg/m(3)的浓度暴露于DE。取雄性仔鼠存活至12周龄，测定雄性生殖器官重量、每日精子生成量(DSP)、血清睾酮水平及性激素合成过程相关因素的mRNA表达。暴露组血清睾酮水平在3wk时显著降低，12wk时显著升高。5wk和12wk时，DSP值也明显降低。组织学检查显示，暴露组生精小管内可见多核巨细胞，生精小管部分空泡化。5wk和12wk时卵泡刺激素受体(FSHR)mRNA和类固醇合成急性调节蛋白(STAR)的表达均显著增加。这项研究表明，产前暴露于DE对子代小鼠精子发生有不利影响。
The effect of prenatal exposure to diesel exhaust (DE) was investigated. Twenty pregnant ICR mice were exposed to DE at the particle concentration of 1.0 mg/m(3), from d 2 until d 16 post-coitum. Male offspring were kept alive until 12 wk of age, and then male reproductive organ weight, daily sperm production (DSP), serum testosterone level, and mRNA expression of sex steroid hormone synthesis process-related factors were measured. Serum testosterone levels of the exposed group were reduced significantly at 3 wk, whereas they were elevated significantly at 12 wk. DSP was also markedly reduced at 5 and 12 wk. Histological examination showed multinucleated giant cells in the seminiferous tubules of the exposed group as well as partial vacuolation of the seminiferous tubules. Follicle-stimulated hormone receptor (FSHR) mRNA expression and steroidogenesis acute regulatory (StAR) protein were significantly increased at 5 wk and 12 wk, respectively. This study suggests that prenatal exposure to DE has detrimental effects of mouse spermatogenesis in offspring.