Immunohistochemical analysis of 3-B-(-) and 7-D-4 epitope expression in canine osteoarthritis.
Immunohistochemical analysis of 3-B-(-) and 7-D-4 epitope expression in canine osteoarthritis.
复制标题
犬骨关节炎中 3-B-(-) 和 7-D-4 表位表达的免疫组织化学分析。
DOI：
10.1002/art.1780361211
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发表时间：
1993
影响因子：
--
通讯作者：
Caterson,B
中科院分区：
文献类型：
--
作者：
Visco,DM;Johnstone,B;Hill,MA;Jolly,GA;Caterson,B
目的：研究3-B-3(-)和7-D-4表位在形态正常和骨关节炎(OA)犬关节软骨中的分布。方法：对9只行颅十字韧带横断术的犬稳定和失稳关节的股骨标本进行免疫组织化学检测。结果：3-B-3(-)和7-D-4表位在发育早期失稳的股胫骨关节软骨浅层表达。两种抗体的染色模式不同，3-B-3(-)阳性反应局限于关节软骨浅层和中上部，7-D-4阳性反应在基质中更为突出，向深层延伸，并随病变进展而增加。这两个表位也在病变周围的浅层和中上部表达，并且在基质丢失之前就可以检测到，蛋白多糖可以通过甲苯胺蓝组织化学染色来鉴定。结论：在本研究中，非典型的硫酸软骨素蛋白多糖在骨关节炎犬的软骨中表达，并且随着病变的进展，表达模式发生了变化。3-B-3(-)和7-D-4表位的出现似乎与实验性骨关节炎软骨退变早期软骨细胞代谢的变化有关。
Objective.To examine the distribution of the 3‐B‐3(–) and 7‐D‐4 epitopes in proteoglycans from morphologically normal and osteoarthritic (OA) canine articular cartilage.Methods.Cartilage samples from the femurs of stable and destabilized stifle joints of 9 dogs that had undergone transection of the cranial cruciate ligament were examined by immunohistochemistry.Results.The 3‐B‐3(–) and 7‐D‐4 epitopes were expressed in the superficial zone of cartilage from the destabilized femorotibial joints in the early stages of developing OA. The staining patterns with these two antibodies differed, with 3‐B‐3(–) reactivity confined to the superficial and upper middle zones of the articular cartilage, and 7‐D‐4 reactivity more prominent in the matrix, extending into the deeper zones and increasing with progression of the lesion. Both epitopes were also expressed in the superficial and upper middle zones of areas peripheral to the lesions and were detectable before the loss of matrix and proteoglycans could be identified by histochemical staining with toluidine blue.Conclusion.In this study, the expression of atypical chondroitin sulfate proteoglycans was demonstrated in osteoarthritic canine cartilage, and the pattern of expression changed as the lesions progressed. The occurrence of 3‐B‐3(–) and 7‐D‐4 epitopes appears to be associated with changes in chondrocyte metabolism in the early stages of cartilage degeneration in experimental osteoarthritis.