Characterization of sleep in Aplysia californica.
Characterization of sleep in Aplysia californica.
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DOI：
10.5665/sleep.3992
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发表时间：
2014-09
期刊：
影响因子：
5.6
通讯作者：
A. Vorster;Harini C. Krishnan;C. Cirelli;Lisa C. Lyons
中科院分区：
文献类型：
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作者：
A. Vorster;Harini C. Krishnan;C. Cirelli;Lisa C. Lyons
研究目的研究海洋软体动物加州海兔的睡眠特征。设计动物的行为和活动通过视频记录来测量活动、休息姿势、休息场所偏好和休息剥夺后的行为。在清醒和休息状态下测量动物的食欲和厌恶刺激的行为反应潜伏期。设置海兔昼夜节律研究实验室。患者或受试者来自太平洋的哈里福尼卡。干预N/A测量和结果海兔几乎完全在夜间以半收缩的身体姿势休息，优先休息的位置在其水箱的上角。与相同时间点的清醒动物相比，休息的动物在头部定位和咬人方面表现出更长的潜伏期，对海藻刺激的反应，以及在对尾巴施加厌恶的盐刺激后较少的逃逸反应步骤。海兔在夜间休息不足后第二天表现出反弹休息，但在白天类似的处理刺激后则不表现出反弹休息。结论海兔的睡眠行为符合无脊椎动物睡眠的所有特征，包括：(1)特定的睡眠体位，(2)首选的休息位置，(3)可逆的行为静止，(4)睡眠中感觉刺激的觉醒阈值提高，(5)睡眠剥夺后的代偿性睡眠反弹。
STUDY OBJECTIVE To characterize sleep in the marine mollusk, Aplysia californica. DESIGN Animal behavior and activity were assessed using video recordings to measure activity, resting posture, resting place preference, and behavior after rest deprivation. Latencies for behavioral responses were measured for appetitive and aversive stimuli for animals in the wake and rest states. SETTING Circadian research laboratory for Aplysia. PATIENTS OR PARTICIPANTS A. californica from the Pacific Ocean. INTERVENTIONS N/A. MEASUREMENTS AND RESULTS Aplysia rest almost exclusively during the night in a semi-contracted body position with preferential resting locations in the upper corners of their tank. Resting animals demonstrate longer latencies in head orientation and biting in response to a seaweed stimulus and less frequent escape response steps following an aversive salt stimulus applied to the tail compared to awake animals at the same time point. Aplysia exhibit rebound rest the day following rest deprivation during the night, but not after similar handling stimulation during the day. CONCLUSIONS Resting behavior in Aplysia fulfills all invertebrate characteristics of sleep including: (1) a specific sleep body posture, (2) preferred resting location, (3) reversible behavioral quiescence, (4) elevated arousal thresholds for sensory stimuli during sleep, and (5) compensatory sleep rebound after sleep deprivation.