Surface Modification of 3D-Poly(glycerol-sebacate)/ Poly(L-lactic-acid) Porous Scaffolds by Oxygen Plasma Enhances Proliferation and Differentiation of Goat Temporomandibular Joint Disc Cells
Surface Modification of 3D-Poly(glycerol-sebacate)/ Poly(L-lactic-acid) Porous Scaffolds by Oxygen Plasma Enhances Proliferation and Differentiation of Goat Temporomandibular Joint Disc Cells
复制标题
氧等离子体对3D-聚(甘油-癸二酸酯)/聚(L-乳酸)多孔支架进行表面修饰可增强山羊颞下颌关节盘细胞的增殖和分化
DOI：
10.1166/jbt.2019.2208
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发表时间：
2019-12
影响因子：
0.1
通讯作者：
康宏
中科院分区：
文献类型：
--
作者：
曹天栋;董芳瑞;张鹏飞;刘静莹;邢喆;包广洁;康宏
3D poly(glycerol-sebacate)/poly(L-lactic-acid) (PGS/PLLA) scaffolds, produced by thermally induced phase separation (TIPS) method were treated with oxygen plasma in order to reduce the hydrophobicity of the PGS/PLLA membranes. After the oxygen plasma treatment, the pore size of scaffolds
became uniform as well as an increasing in the roughness of the surface. No influence on chemical characteristics such as composition and bond types of PLLA was detected after the treatment. However, the change in the ratio of the scaffold elements before and after the oxygen plasma treatment
was detected. Oxygen plasma treatment reduced the average of water contact angle of the TIPS film from 103.7± 10.1 to 64.7± 12.6 at the maximum exposure time (970 s) and power (100 W). The results showed that the goat temporomandibular joint (TMJ) cells attached well to the surface
of the oxygen plasma modified scaffold. Furthermore, this modification significantly enhanced cell viability, increased cell proliferation and expression of related genes (Collagen type I mRNA and Collagen type II mRNA). The results demonstrated that it is a promising modification method to
promote applications of PGS/PLLA based scaffolds to the regeneration of TMJ discs.
