Nicotine enhances GABAergic inhibition of oxytocin mRNA-expressing neuron in the hypothalamic paraventricular nucleus in vitro in rats
Nicotine enhances GABAergic inhibition of oxytocin mRNA-expressing neuron in the hypothalamic paraventricular nucleus in vitro in rats
复制标题
尼古丁在体外增强大鼠下丘脑室旁核中表达催产素 mRNA 的神经元的 GABA 抑制。
DOI：
10.1016/j.neulet.2016.12.005
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发表时间：
2017-01
影响因子：
2.5
通讯作者：
Qiu De-Lai
中科院分区：
文献类型：
--
作者：
Zhang Ke-Min;Zhao Guo-Yan;Zhang Bin-Bin;Xu Qi;Chu Chun-Ping;Jin Hua;Qiu De-Lai
我们最近发现，细胞外给予尼古丁间接兴奋下丘脑室旁核（PVN）促肾上腺皮质激素释放激素（CRH）mRNA表达神经元。本实验采用全细胞膜片钳技术、免疫组织化学方法和单细胞逆转录多重聚合酶链反应（SC-RT-mPCR）方法，研究了尼古丁对大鼠PVN催产素（OT）mRNA表达的影响。结果表明，在92个PVN大细胞神经元中，有79.3%（73/92）的神经元同时表达GAPDH和OT mRNA。在电流钳记录条件下，局部微量应用尼古丁（1-300 μM）可引起76.7%（56/73）的PVN OT mRNA表达大细胞神经元自发放电频率降低，并伴有膜电位超极化。尼古丁对PVN OT mRNA表达大细胞神经元自发活动的抑制作用呈剂量依赖性。半抑制浓度（IC 50）为2.9 μM。尼古丁诱导的PVN OT mRNA表达的大细胞神经元超极化对GABA A受体拮抗剂SR95531（10 μM）和河豚毒素（TTX，1 μM）敏感。此外，局部微量应用尼古丁诱导的自发抑制性突触后电位（sIPSPs）的频率显着增加，但没有变化的sEPSPs振幅的OT-mRNA表达神经元。Biocytin染色证实尼古丁敏感的OT-mRNA表达神经元为PVN大细胞神经元。这些结果表明，尼古丁增强GABA能抑制，导致PVN OT-mRNA表达神经元的自发放电率降低。这些结果表明，尼古丁通过增强突触前动作电位驱动和GABA量子释放来调节PVN OT分泌。
We recently found that extracellular administration of nicotine indirectly excited hypothalamic paraventricular nucleus (PVN) corticotropin-releasing hormone (CRH) mRNA-expressing neurons. In this study, we studied the effect of nicotine on PVN oxytocin (OT) mRNA-expressing neuronin vitroin rats, by whole-cell patch-clamp recording technique, immunohistochemistry methods and single-cell reverse-transcription multiplex polymerase chain reaction (SC-RT-mPCR) methods Our results showed that 79.3% (73/92) of the 92 PVN putative magnocellular neurons co-expressed GAPDH mRNA and OT mRNA. Under current-clamp recording conditions, local micro application of nicotine (1–300 μM) induced a decrease in spontaneous firing rate accompanied with a hyperpolarization of membrane potential in 76.7% (56/73) of PVN OT mRNA-expressing magnocellular neurons. The nicotine induced inhibition in spontaneous activity of PVN OT mRNA-expressing magnocellular neurons was dose-dependent. The half-inhibitory concentration (IC50) is 2.9 μM. The nicotine induced hyperpolarization of PVN OT mRNA-expressing magnocellular neurons was sensitive to GABAAreceptor antagonist, SR95531 (10 μM) and tetrodotoxin (TTX, 1 μM). In addition, local micro application of nicotine induced a significant increase in frequency of spontaneous inhibitory postsynaptic potentials (sIPSPs), but without changes in the sEPSPs amplitude of the OT-mRNA expressing neurons. Biocytin staining confirmed that the nicotine-sensitive OT-mRNA expressing neurons were the PVN magnocellular neurons. These results demonstrated that nicotine enhances the GABAergic inhibition, resulting in a decrease in spontaneous firing rate of the PVN OT-mRNA expressing neurons. These findings suggested that nicotine modulated PVN OT secretion via enhancement of both presynaptic action potential drive and quantal GABA release.
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影响因子：
2.5
作者：
Qiu, DL;Chu, CP;Kannan, H
通讯作者：
Kannan, H
影响因子：
3.3
作者：
M. Zaninetti;E. Tribollet;D. Bertrand;M. Raggenbass
通讯作者：
M. Zaninetti;E. Tribollet;D. Bertrand;M. Raggenbass
影响因子：
3.7
作者：
Matta, SG;Fu, YT;Sharp, BM
通讯作者：
Sharp, BM
影响因子：
5.5
作者：
Park, Jin Bong;Skalska, Silvia;Stern, Javier E.
通讯作者：
Stern, Javier E.
影响因子：
4.7
作者：
Yu G;Sharp BM
通讯作者：
Sharp BM