Protective roles of cornuside in acute myocardial ischemia and reperfusion injury in rats
Protective roles of cornuside in acute myocardial ischemia and reperfusion injury in rats
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DOI：
10.1016/j.phymed.2010.07.009
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发表时间：
2011-02-15
期刊：
影响因子：
7.9
通讯作者：
Liu, Hong-Zhan
中科院分区：
文献类型：
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作者：
Jiang, Wang-Lin;Zhang, Shu-Min;Liu, Hong-Zhan
角苷是从药用玉米果实中分离得到的一种环烯醚萜糖苷。Et Zucc.在本研究中，我们观察了山茱萸苷在体抗心肌缺血再灌注(I/R)损伤的作用，并阐明了其可能的机制。采用冠脉结扎后再灌流或异丙肾上腺素(ISO)诱导大鼠心肌OR模型，山茱萸皂苷(20和40 mg/kg)静脉注射。保护动物免受心肌I/R损伤，表现为心肌梗死体积减少，血流动力学改善，心肌损伤严重程度减轻。Cornuside治疗还可减轻中性粒细胞(PMN)的浸润，降低心脏髓过氧化酶(MPO)活性，降低血清促炎因子水平，并减少心脏中磷酸化的I-kappaB-α和核因子kappaB(NF-kappa B)水平。此外，山茱萸皂苷还具有显著的抗氧化活性，并能抑制异丙肾上腺素诱导的心肌细胞坏死。因此，山茱萸苷对大鼠心肌I/R损伤具有保护作用，其机制可能与其抗炎作用有关。这些结果提示，角苷可用于炎症和氧化损伤突出的心肌I/R的治疗。(C)2010年爱思唯尔股份有限公司。版权所有。
Cornuside is a secoiridoid glucoside isolated from the fruit of Corn us officinalis SIEB. et ZUCC. In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effects of cornuside in vivo and elucidated the potential mechanism. Rat models of myocardial OR were induced by coronary occlusion followed by reperfusion or by Isoproterenol (ISO), treatment of rats with cornuside (20 and 40 mg/kg, i.v.) protected the animals from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics and reduction of myocardial damage severity. Treatment with cornuside also attenuated polymorphonuclear leukocytes (PMNs) infiltration, decreased myeloperoxidase (MPO) activity in the heart, lowered serum levels of pro-inflammatory factors and reduced phosphorylated I kappa B-alpha and nuclear factor kappa B (NF-kappa B) levels in the heart. Additionally, cornuside was shown to have remarkable antioxidant activity and inhibited ISO-induced myocardial cell necrosis. Thus, cornuside appeared to protect the rat from myocardial I/R injury by acting as an anti-inflammatory agent. These findings suggested that cornuside may be used therapeutically in the setting of myocardial I/R where inflammation and oxidant injury are prominent. (C) 2010 Elsevier GmbH. All rights reserved.