Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP.
Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP.
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大鼠肝葡萄糖-6-磷酸酶催化亚基 cDNA 的分离：胰岛素、地塞米松和 cAMP 对FAO 肝癌细胞中基因表达的调节。
DOI：
10.1006/bbrc.1994.1702
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发表时间：
1994
影响因子：
3.1
通讯作者：
Pilkis,SJ
中科院分区：
文献类型：
--
作者：
Lange,AJ;Argaud,D;el-Maghrabi,MR;Pan,W;Maitra,SR;Pilkis,SJ
从λ gt 11噬菌体的大鼠肝cDNA文库中分离编码大鼠肝葡萄糖-6-磷酸酶催化亚基的cDNA克隆（EC 3.1.3.9）。该cDNA序列和由此衍生的氨基酸序列与小鼠和人肝葡萄糖-6-磷酸酶形式的相应序列具有90%以上的同一性。北方印迹分析显示，地塞米松诱导FAO肝癌细胞的葡萄糖-6-磷酸酶mRNA的表达，而胰岛素抑制其表达。当两种激素加在一起时，胰岛素完全抑制了糖皮质激素的作用。cAMP单独添加降低了葡萄糖-6-磷酸酶mRNA的丰度。结果表明，多激素调节肝葡萄糖-6-磷酸酶的基因表达，并支持胰岛素的主导作用。
cDNA clones coding for the catalytic subunit of rat liver glucose-6-phosphatase (EC 3.1.3.9) were isolated from a rat liver cDNA library in lambda gt11phage. The sequence of the cDNA and the amino acid sequence derived from it were greater than 90% identical to the corresponding sequences for the mouse and human forms of liver glucose-6-phosphatase. Northern blot analysis of RNA from FAO hepatoma cells revealed that dexamethasone induced the glucose-6-phosphatase mRNA while insulin suppressed its expression. When both hormones were added together insulin completely suppressed the effect of glucocorticoid. cAMP addition alone decreased the abundance of glucose-6-phosphatase mRNA. The results demonstrate multihormonal regulation of gene expression of hepatic glucose-6-phosphatase and support a dominant role for insulin.