Cell death in normal and rough eye mutants of Drosophila.
Cell death in normal and rough eye mutants of Drosophila.
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作者：
T. Wolff;D. Ready
果蝇复合眼的常规，重复的细胞模式使其成为细胞死亡中缺陷的敏感放大器。定量和组织学方法揭示了35至50 h发育之间的细胞死亡相位，该阶段消除了每个羊皮的2至3个剩余细胞之间。该时期的时机与细胞死亡是一致的，这是在建立Ommatidium的细胞命运的渐进序列中指定的最后一个命运。细胞死亡的超微结构调查表明，蝇眼中的垂死细胞与编程细胞死亡的标准描述具有相似性以及差异。细胞死亡无法去除多余细胞的失败会使视网膜晶格混乱。粗糙眼突变体的筛选鉴定出正常消除视网膜上皮细胞所需的两个基因，最粗糙和echinus。将增强子陷阱用作细胞谱系标记的使用表明，与其他视网膜细胞一样，圆锥细胞与彼此或其邻居无关。
The regular, reiterated cellular pattern of the Drosophila compound eye makes it a sensitive amplifier of defects in cell death. Quantitative and histological methods reveal a phase of cell death between 35 and 50 h of development which removes between 2 and 3 surplus cells per ommatidium. The timing of this epoch is consistent with cell death as the last fate to be specified in the progressive sequence of cell fates that build the ommatidium. An ultrastructural survey of cell death suggests dying cells in the fly eye have similarities as well as differences with standard descriptions of programmed cell death. A failure of cell death to remove surplus cells disorganizes the retinal lattice. A screen of rough eye mutants identifies two genes, roughest and echinus, required for the normal elimination of cells from the retinal epithelium. The use of an enhancer trap as a cell lineage marker shows that the cone cells, like other retinal cells, are not clonally related to each other or to their neighbors.